Recombinant Human Alpha-1,3/1,6-mannosyltransferase ALG2 (ALG2)

Code CSB-CF863983HU
MSDS
Size Pls inquire
Source in vitro E.coli expression system
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Product Details

Target Names
ALG2
Uniprot No.
Alternative Names
ALG2; UNQ666/PRO1298; Alpha-1,3/1,6-mannosyltransferase ALG2; Asparagine-linked glycosylation protein 2 homolog; GDP-Man:Man(1GlcNAc(2-PP-Dol alpha-1,3-mannosyltransferase; GDP-Man:Man(1GlcNAc(2-PP-dolichol mannosyltransferase; GDP-Man:Man(2GlcNAc(2-PP-Dol alpha-1,6-mannosyltransferase
Species
Homo sapiens (Human)
Expression Region
1-416
Target Protein Sequence
MAEEQGRERDSVPKPSVLFLHPDLGVGGAERLVLDAALALQARGCSVKIWTAHYDPGHCF AESRELPVRCAGDWLPRGLGWGGRGAAVCAYVRMVFLALYVLFLADEEFDVVVCDQVSAC IPVFRLARRRKKILFYCHFPDLLLTKRDSFLKRLYRAPIDWIEEYTTGMADCILVNSQFT AAVFKETFKSLSHIDPDVLYPSLNVTSFDSVVPEKLDDLVPKGKKFLLLSINRYERKKNL TLALEALVQLRGRLTSQDWERVHLIVAGGYDERVLENVEHYQELKKMVQQSDLGQYVTFL RSFSDKQKISLLHSCTCVLYTPSNEHFGIVPLEAMYMQCPVIAVNSGGPLESIDHSVTGF LCEPDPVHFSEAIEKFIREPSLKATMGLAGRARVKEKFSPEAFTEQLYRYVTKLLV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Mannosylates Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate.
Gene References into Functions
  1. We identify ALG14 and ALG2 as novel genes in which mutations cause a congenital myasthenic syndrome PMID: 23404334
  2. The ALG2 binding site is necessary for the punctate distribution of the carboxyl-terminal proline-rich region of Alix in HeLa cells. PMID: 14999017
  3. The expression and transport of ALG-2 in association with TSG101 and Vps4B are reported. PMID: 16004603
Involvement in disease
Congenital disorder of glycosylation 1I (CDG1I); Myasthenic syndrome, congenital, 14 (CMS14)
Subcellular Location
Membrane; Single-pass membrane protein.
Protein Families
Glycosyltransferase group 1 family, Glycosyltransferase 4 subfamily
Database Links

HGNC: 23159

OMIM: 607905

KEGG: hsa:85365

STRING: 9606.ENSP00000417764

UniGene: Hs.40919

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