Recombinant Human Long-chain-fatty-acid--CoA ligase 4 (ACSL4)

Code CSB-CF001193HU
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Source in vitro E.coli expression system
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Product Details

Target Names
Uniprot No.
Alternative Names
ACSL4; ACS4; FACL4; LACS4; Long-chain-fatty-acid--CoA ligase 4; Arachidonate--CoA ligase; Long-chain acyl-CoA synthetase 4; LACS 4
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Please contact us to get it.

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Target Background

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.
Gene References into Functions
  1. The regulatory network among peroxisomal ABCD2:ACSL4:VLCFA serves as a novel regulator of cartilage homeostasis, and these data may provide novel insights into the role of peroxisomal fatty acid metabolism in pathogenesis of human osteoarthritis (OA). PMID: 30264402
  2. Study demonstrated that ACSL4 was overexpressed in HCC. PMID: 28887439
  3. studies have identified a novel substrate-induced posttranslational regulatory mechanism by which AA downregulates ACSL4 protein expression in hepatic cells. PMID: 24879802
  4. Data show that ELOVL7, SOCS3, ACSL4 and CLU were upregulated while PRKAR1A and ABCG1 were downregulated in the phlegm-dampness group. PMID: 27928700
  5. ACSL4 is not only a sensitive monitor of ferroptosis, but also an important contributor of ferroptosis. PMID: 27565726
  6. Silencing of ACSL4 eliminated the 17beta-estradiol-induced increase in AA and EPA uptake. PMID: 28334272
  7. ACSL4 plays a tumor-suppressive role in gastric cancer. PMID: 26949059
  8. Suggest role for ACSL4 expression in development of castration-resistant prostate cancer. PMID: 26636648
  9. we demonstrate that ACSL4 can be considered a novel activator of the mTOR pathway PMID: 26536660
  10. In vitro analysis showed that a recombinant COX-2 enzyme more effectively metabolized 5(S)-HETE to 5-11-diHETE compared to COX-1 enzyme. PMID: 26282205
  11. Upregulation of ACSL4 is responsible for the increase in triacylglycerol species containing long polyunsaturated fatty acids during activation of hepatic stellate cells. PMID: 25500141
  12. Report PPARdelta-mediated regulatory mechanism for ACSL4 expression in liver tissue and cultured hepatic cells. PMID: 25645621
  13. ACSL4 can serve as both a biomarker for, and mediator of, an aggressive breast cancer phenotype. PMID: 24155918
  14. This study found no significant relationship between FACL4 and cognitive function. PMID: 20452052
  15. conclude that in our model system exogenous fatty acids are channeled preferentially towards phosphatidylinositol by ACSL4 overexpression PMID: 24201376
  16. MiR-205 down-regulates ACSL4 through targeting its 3'UTR in hepatoma cells. PMID: 24576478
  17. The functional interaction between Acyl-CoA synthetase 4, 5-lipooxygenase and cyclooxygenase-2 controls tumor growth. PMID: 22808264
  18. the involvement of SHP2 activity in the regulation of the expression of the fatty acid-metabolizing enzyme ACSL4 PMID: 21903867
  19. A total of six novel and 11 known single nucleotide polymorphisms were identified. Further studies are warranted to analyze the candidate genes at Xq11.1-q21.33. PMID: 21384559
  20. CSL4 modulates PGE release from human erial smooth muscle cells. PMID: 21242590
  21. Data suggest that the development of combinatory therapies that profit from the ACSL4, lipooxygenase and COX-2 synergistic action may allow for lower medication doses and avoidance of side effects. PMID: 21085606
  22. Fatty acid CoA ligase 4 is up-regulated in colon adenocarcinoma. PMID: 11731423
  23. FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation PMID: 11889465
  24. FACL4 is highly expressed in hippocampal and cerebellar neurons and may have a role in X linked mental retardation. PMID: 12525535
  25. Overexpression of Fatty acid-CoA ligase 4 is associated with human hepatocellular carcinoma PMID: 12824887
  26. FACL4 is involved in the hepatocellular carcinoma tumorigenesis and both cAMP and p38 MAPK pathways are associated with the regulation of FACL4 PMID: 15849811
  27. Disruption of DMD and the absence of ACSL4 in a patient are responsible for neuromuscular disease and cognitive impairment. PMID: 16276108
  28. FACL4 affects HCC cell growth and suggest that modulation of FACL4 expression/activity is an approach for treatment of HCC. PMID: 17934335
  29. FACL4 might play a role in the growth of hepatic cancer cells. PMID: 18059177
  30. No association between polymorphisms in the FACL4 (fatty acid-CoA ligase 4) gene and nonspecific mental retardation in Qin-Ba mountain region of China. PMID: 18614287
  31. The common SNP (C to T substitution) in the first intron of the FACL4 gene is associated with altered Fatty Acid composition of plasma phosphatidylcholines in patients with metabolic syndrome . PMID: 19346733
  32. ACSL4 can substitute the functions of dAcsl(l(2)44DEa in organismal viability, lipid storage and the neural wiring in visual center. PMID: 19617635

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Involvement in disease
Mental retardation, X-linked 63 (MRX63); Alport syndrome with mental retardation, midface hypoplasia and elliptocytosis (ATS-MR)
Subcellular Location
Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. Cell membrane.
Protein Families
ATP-dependent AMP-binding enzyme family
Database Links

HGNC: 3571

OMIM: 300157

KEGG: hsa:2182

STRING: 9606.ENSP00000339787

UniGene: Hs.268785

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