ACSL4 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA583012A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(20µg), Hela whole cell lysate(20µg),COLO-205 whole cell lysate(20µg), A549 whole cell lysate(20µg), U-87MG whole cell lysate(20µg),LO2 whole cell lysate(20µg), HEK293 whole cell lysate(20µg) All lanes: ACSL4 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 79 kDa Observed band size: 79 kDa Exposure time：40s Immunofluorescence staining of A-549 cell with CSB-RA583012A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing HEK293T cells stained with CSB-RA583012A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Target Background

Product Details

Uniprot No.

O60488

Target Names

ACSL4

Alternative Names

ACS 4 antibody; ACS4 antibody; ACSL 4 antibody; Acsl4 antibody; ACSL4_HUMAN antibody; acyl CoA synthetase 4 antibody; Acyl CoA synthetase long chain family member 4 antibody; FACL 4 antibody; FACL4 antibody; Fatty acid Coenzyme A ligase antibody; fatty acid Coenzyme A ligase long-chain 4 antibody; LACS 4 antibody; LACS4 antibody; Lignoceroyl CoA synthase antibody; Long chain 4 antibody; long chain acyl CoA synthetase 4 antibody; long chain fatty acid CoA ligase 4 antibody; long chain fatty acid Coenzyme A ligase 4 antibody; Long-chain acyl-CoA synthetase 4 antibody; Long-chain-fatty-acid--CoA ligase 4 antibody; MRX63 antibody; MRX68 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human ACSL4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

15G3

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ACSL4 (Acyl-CoA Synthetase Long Chain Family Member 4) plays a critical role in lipid metabolism by catalyzing the conversion of long-chain fatty acids into fatty acyl-CoA esters, a process essential for membrane phospholipid remodeling. This enzyme has gained significant research attention due to its established role as a key driver of ferroptosis, the iron-dependent form of regulated cell death. ACSL4 enriches cellular membranes with polyunsaturated fatty acids, making them susceptible to lipid peroxidation, which positions this protein as a central focus in cancer biology, neurodegeneration, and metabolic disease research.

This recombinant monoclonal antibody, generated in rabbit using clone 15G3, offers the reproducibility and sequence-defined consistency that demanding experimental workflows require. Unlike traditional hybridoma-derived antibodies, recombinant production ensures lot-to-lot uniformity, allowing you to maintain confidence in your results across extended studies and collaborative projects.

Validation data demonstrates robust performance across multiple applications. Western blot analysis detects a band at the predicted 79 kDa molecular weight across a diverse panel of human cell lines including HepG2, HeLa, COLO-205, A549, U-87MG, LO2, and HEK293, confirming reliable detection in hepatic, cervical, colorectal, lung, and glioblastoma models. Immunofluorescence staining in A-549 cells reveals clear cytoplasmic localization patterns, while flow cytometry analysis in HEK293T cells shows distinct positive population shifts compared to isotype controls.

This antibody serves researchers investigating ferroptosis mechanisms, lipid metabolism disorders, and cancer cell vulnerability to oxidative stress, providing a dependable tool for characterizing ACSL4 expression and localization in human experimental systems.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs. Modulates prostaglandin E2 secretion.

Gene References into Functions

The regulatory network among peroxisomal ABCD2:ACSL4:VLCFA serves as a novel regulator of cartilage homeostasis, and these data may provide novel insights into the role of peroxisomal fatty acid metabolism in pathogenesis of human osteoarthritis (OA). PMID: 30264402
Study demonstrated that ACSL4 was overexpressed in HCC. PMID: 28887439
studies have identified a novel substrate-induced posttranslational regulatory mechanism by which AA downregulates ACSL4 protein expression in hepatic cells. PMID: 24879802
Data show that ELOVL7, SOCS3, ACSL4 and CLU were upregulated while PRKAR1A and ABCG1 were downregulated in the phlegm-dampness group. PMID: 27928700
ACSL4 is not only a sensitive monitor of ferroptosis, but also an important contributor of ferroptosis. PMID: 27565726
Silencing of ACSL4 eliminated the 17beta-estradiol-induced increase in AA and EPA uptake. PMID: 28334272
ACSL4 plays a tumor-suppressive role in gastric cancer. PMID: 26949059
Suggest role for ACSL4 expression in development of castration-resistant prostate cancer. PMID: 26636648
we demonstrate that ACSL4 can be considered a novel activator of the mTOR pathway PMID: 26536660
In vitro analysis showed that a recombinant COX-2 enzyme more effectively metabolized 5(S)-HETE to 5-11-diHETE compared to COX-1 enzyme. PMID: 26282205
Upregulation of ACSL4 is responsible for the increase in triacylglycerol species containing long polyunsaturated fatty acids during activation of hepatic stellate cells. PMID: 25500141
Report PPARdelta-mediated regulatory mechanism for ACSL4 expression in liver tissue and cultured hepatic cells. PMID: 25645621
ACSL4 can serve as both a biomarker for, and mediator of, an aggressive breast cancer phenotype. PMID: 24155918
This study found no significant relationship between FACL4 and cognitive function. PMID: 20452052
conclude that in our model system exogenous fatty acids are channeled preferentially towards phosphatidylinositol by ACSL4 overexpression PMID: 24201376
MiR-205 down-regulates ACSL4 through targeting its 3'UTR in hepatoma cells. PMID: 24576478
The functional interaction between Acyl-CoA synthetase 4, 5-lipooxygenase and cyclooxygenase-2 controls tumor growth. PMID: 22808264
the involvement of SHP2 activity in the regulation of the expression of the fatty acid-metabolizing enzyme ACSL4 PMID: 21903867
A total of six novel and 11 known single nucleotide polymorphisms were identified. Further studies are warranted to analyze the candidate genes at Xq11.1-q21.33. PMID: 21384559
CSL4 modulates PGE release from human erial smooth muscle cells. PMID: 21242590
Data suggest that the development of combinatory therapies that profit from the ACSL4, lipooxygenase and COX-2 synergistic action may allow for lower medication doses and avoidance of side effects. PMID: 21085606
Fatty acid CoA ligase 4 is up-regulated in colon adenocarcinoma. PMID: 11731423
FACL4, encoding fatty acid-CoA ligase 4, is mutated in nonspecific X-linked mental retardation PMID: 11889465
FACL4 is highly expressed in hippocampal and cerebellar neurons and may have a role in X linked mental retardation. PMID: 12525535
Overexpression of Fatty acid-CoA ligase 4 is associated with human hepatocellular carcinoma PMID: 12824887
FACL4 is involved in the hepatocellular carcinoma tumorigenesis and both cAMP and p38 MAPK pathways are associated with the regulation of FACL4 PMID: 15849811
Disruption of DMD and the absence of ACSL4 in a patient are responsible for neuromuscular disease and cognitive impairment. PMID: 16276108
FACL4 affects HCC cell growth and suggest that modulation of FACL4 expression/activity is an approach for treatment of HCC. PMID: 17934335
FACL4 might play a role in the growth of hepatic cancer cells. PMID: 18059177
No association between polymorphisms in the FACL4 (fatty acid-CoA ligase 4) gene and nonspecific mental retardation in Qin-Ba mountain region of China. PMID: 18614287
The common SNP (C to T substitution) in the first intron of the FACL4 gene is associated with altered Fatty Acid composition of plasma phosphatidylcholines in patients with metabolic syndrome . PMID: 19346733
ACSL4 can substitute the functions of dAcsl(l(2)44DEa in organismal viability, lipid storage and the neural wiring in visual center. PMID: 19617635

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Involvement in disease

Mental retardation, X-linked 63 (MRX63); Alport syndrome with mental retardation, midface hypoplasia and elliptocytosis (ATS-MR)

Subcellular Location

Mitochondrion outer membrane; Single-pass type III membrane protein. Peroxisome membrane; Single-pass type III membrane protein. Microsome membrane; Single-pass type III membrane protein. Endoplasmic reticulum membrane; Single-pass type III membrane protein. Cell membrane.

Protein Families

ATP-dependent AMP-binding enzyme family

Database Links

HGNC: 3571

OMIM: 300157

KEGG: hsa:2182

STRING: 9606.ENSP00000339787

UniGene: Hs.268785

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PPAR signaling pathway Adipocytokine signaling pathway Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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