Recombinant Human Nucleolar transcription factor 1 (UBTF)

Code CSB-CF025524HU
Size $878
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
UBTF
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
UBTF; UBF; UBF1; Nucleolar transcription factor 1; Autoantigen NOR-90; Upstream-binding factor 1; UBF-1
Species
Homo sapiens (Human)
Source
in vitro E.coli expression system
Expression Region
1-764aa
Target Protein Sequence
MNGEADCPTDLEMAAPKGQDRWSQEDMLTLLECMKNNLPSNDSSKFKTTESHMDWEKVAFKDFSGDMCKLKWVEISNEVRKFRTLTELILDAQEHVKNPYKGKKLKKHPDFPKKPLTPYFRFFMEKRAKYAKLHPEMSNLDLTKILSKKYKELPEKKKMKYIQDFQREKQEFERNLARFREDHPDLIQNAKKSDIPEKPKTPQQLWYTHEKKVYLKVRPDATTKEVKDSLGKQWSQLSDKKRLKWIHKALEQRKEYEEIMRDYIQKHPELNISEEGITKSTLTKAERQLKDKFDGRPTKPPPNSYSLYCAELMANMKDVPSTERMVLCSQQWKLLSQKEKDAYHKKCDQKKKDYEVELLRFLESLPEEEQQRVLGEEKMLNINKKQATSPASKKPAQEGGKGGSEKPKRPVSAMFIFSEEKRRQLQEERPELSESELTRLLARMWNDLSEKKKAKYKAREAALKAQSERKPGGEREERGKLPESPKRAEEIWQQSVIGDYLARFKNDRVKALKAMEMTWNNMEKKEKLMWIKKAAEDQKRYERELSEMRAPPAATNSSKKMKFQGEPKKPPMNGYQKFSQELLSNGELNHLPLKERMVEIGSRWQRISQSQKEHYKKLAEEQQKQYKVHLDLWVKSLSPQDRAAYKEYISNKRKSMTKLRGPNPKSSRTTLQSKSESEEDDEEDEDDEDEDEEEEDDENGDSSEDGGDSSESSSEDESEDGDENEEDDEDEDDDEDDDEDEDNESEGSSSSSSSSGDSSDSDSN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
93.4kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Gene References into Functions
  1. TP53INP2 promotes ribosome biogenesis through facilitating rRNA synthesis at the nucleolus PMID: 27172002
  2. Study demonstrated that HMG box 1, the linker region between the HMG boxes and C-terminal acidic region (AR), and the AR cooperatively regulate the nucleolar localization of UBF. PMID: 28874518
  3. In seven unrelated affected individuals, all suffering from developmental regression starting at 2.5-7 years, the authors identified a heterozygous variant, c.628G>A in UBTF, encoding p.Glu210Lys in UBF, which occurred de novo in all cases. PMID: 28777933
  4. RINT-1 interacts with MSP58 and UBF within nucleoli and plays a role in ribosomal gene transcription. PMID: 27530925
  5. This study provides some compelling evidences in support of HBx-elicited and c-Myc-mediated increase in UBF levels that abets oncogenic onslaught in hepatic cells by increasing rDNA transcription and ribosome biogenesis. PMID: 25890091
  6. These results suggest that UBF can inhibit gene expression from viral DNA prior to its replication. PMID: 25965800
  7. we have uncovered a novel role for UBTF1 and UBTF2 in maintaining genome stability through coordinating the expression of highly transcribed Pol I (UBTF1 activity) and Pol II genes (UBTF2 activity). PMID: 25452314
  8. p14ARF fails to prevent E7-mediated UBF1 phosphorylation, but could facilitate nucleolar pRb inactivation by targeting E7 to the nucleolus. PMID: 24798431
  9. PIP2 depletion reduces Pol I transcription. PMID: 23591814
  10. UBF is expressed widely by human multiple fetal tissue,s and the expression level is very high in HL-60 cells. PMID: 21162312
  11. Downregulation of RUNX2 expression reduces the localization of HDAC1 to the nucleolar periphery and also decreases the association between HDAC1 and UBF. PMID: 22393235
  12. hALP binds the upstream binding factor (UBF) in vivo and in vitro PMID: 21177859
  13. depletion of upstream binding factor (UBF; an rRNA transcription factor) decreased the chromatin binding affinity of B23, which in turn led to an increase in histone density at the r-chromatin PMID: 20713446
  14. The three-dimensional solution structure of the first high mobility group (HMG) box domain in upstream binding factor has been determined by multidimensional NMR. PMID: 11969401
  15. we have identified an interaction between UBF and TAF1, a factor involved in the transcription of cell cycle and growth regulatory genes. Coimmunoprecipitation and protein-protein interaction assays confirmed that TAF1 binds to UBF. PMID: 12498690
  16. The DNA binding affinity of UBF's fifth box domain (HMG box 5) is found to be much weaker than that of the first HMG box domain (HMG box 1). PMID: 12590579
  17. Data show that both UBF1 and UBF2 activate RNA polymerase II-regulated, beta-catenin-responsive promoters. PMID: 12748295
  18. Proto-Oncogene Proteins c-myc activated transcription from the UBF promoter PMID: 15282543
  19. results suggest that extensive binding of UBF is responsible for formation and maintenance of the secondary constriction at active NORs, and that UBF mediates recruitment of the pol I machinery to nucleoli independently of promoter elements PMID: 15598984
  20. Data suggest that the A state of human upstream binding factor HMG Box1 could represent a potential folding intermediate on protein folding pathway. PMID: 15752694
  21. A potential protein-folding pathway is proposed for upstream binding factor HMG box 1 domain based on the early stages of its pH 2.1 unfolded state characterized by multidimensional heteronuclear magnetic resonance spectroscopy. PMID: 15924431
  22. SL1 directs preinitiation complex formation, functioning in core promoter binding, RNA polymerase I recruitment, and UBF stabilization. PMID: 15970593
  23. model for CAST/hPAF49 function in which the network of interactions of Pol I-specific subunits with UBF facilitates conformational changes of the polymerase, leading to stabilization of the Pol I-template complex and, thereby, activation of transcription PMID: 16809778
  24. UBF activates transcription in the transition between initiation and elongation, at promoter escape by RNA polymerase I (Pol I). PMID: 16858408
  25. Upon p14ARF overexpression, UBF was found hypophosphorylated, thus unable to efficiently recruit the transcription complex, these data define a new p53-independent pathway that could regulate cell cycle through the negative control of rRNA transcription. PMID: 16924243
  26. GdmCl-induced equilibrium unfolding transition of HMG box 5 of hUBF was monitored by both circular dichroism and fluorescence spectra. A cooperative two-state unfolding process was observed PMID: 17260958
  27. possible sites in hUBF HMG box 5 that may interact with the first bromodomain of TAF1 were proposed PMID: 17505112
  28. transcription factor UBF binds extensively across rDNA throughout the cell cycle, resulting in a specialized form of chromatin that is the hallmark of active nucleolar organizer regions PMID: 17699751
  29. found a small but significant difference between the emerging daughter cells in the number of UBF-loaded NORs PMID: 18502146
  30. The authors propose that UBF is recruited to the replication compartments to aid replication of HSV-1 DNA. PMID: 19088274
  31. The established differential localization of UBF in nucleoli of HeLa cells has a functional meaning. It reflects both the level of rRNA synthesis activities and the architectural role of UBF in nucleoli of these cells. PMID: 19393134
  32. these results implicate a fluid helix-turn-helix folding model of hUBF Box-5. PMID: 19452555
  33. central repeated domain of treacle binds with RNA polymerase I, while that the treacle C-terminus is involved in rDNA promoter recognition and UBF recruitment. PMID: 19527688

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Subcellular Location
Nucleus, nucleolus.
Database Links

HGNC: 12511

OMIM: 600673

KEGG: hsa:7343

STRING: 9606.ENSP00000302640

UniGene: Hs.89781

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