Webinar: Solving Western Blot Challenges: From Pitfalls to Publication-Ready Data

Western blotting (WB) remains the gold standard for identifying and quantifying specific proteins within complex biological samples, such as cell lysates or tissue extracts. By integrating SDS-PAGE-based separation, precise membrane transfer, and highly specific antibody detection, this technique delivers critical insights into protein expression profiles, post-translational modifications, and molecular weight validation—data indispensable for advancing functional genomics, elucidating disease mechanisms (e.g., cancer biomarkers, neurodegenerative pathways), and accelerating therapeutic discovery. Recent innovations, including multiplex fluorescent detection and automated platforms, have further expanded WB's capabilities into high-throughput and quantitative applications, revolutionizing its role in both research and clinical diagnostics.

Despite its versatility, WB experiments are prone to technical challenges—inconsistent band patterns, high background noise, or weak signals—often stemming from sub-optimal reagent selection, protocol variability, or inadequate quality controls. Addressing these issues demands a systematic approach combining optimized workflows with rigorous validation. In this webinar, we will recognizing common pitfalls and adopt tailored strategies to address them, ensuring reliability and accelerating your path to robust, publishable results.

Key Points

1. Western blotting as the "Gold Standard" in Protein Analysis

2. Experimental considerations, critical variables and problem-solving strategies

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Speaker

Ying Zhou PhD

Product Manager, CUSABIO

Dr. Ying Zhou is a Product Manager at CUSABIO, responsible for product marketing, development, and optimization. Before joining CUSABIO, Dr. Zhou earned her Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute in the United States. Following her graduation, she pursued post-doctoral training at the University of California, Davis. Dr. Zhou's expertise lies in molecular biology and protein science, specifically in mRNA expression and regulation. She is particularly interested in mRNA stability and RNA-interacting proteins.