Product Details

Target Name

platelet factor 4

Alternative Names

Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit

Abbreviation

PF4

Uniprot No.

Q9Z126

Species

Mus musculus (Mouse)

Sample Types

serum, plasma, tissue homogenates

Detection Range

3.125 ng/mL-200 ng/mL

Sensitivity

1.603 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	86
	Range %	81-90
1:400	Average %	98
	Range %	92-104
1:800	Average %	97
	Range %	94-102
1:1600	Average %	94
	Range %	89-98

Recovery

The recovery of mouse PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	86-94
EDTA plasma (n=4)	98	92-104

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.501	2.402	2.452	2.308
100	2.093	1.975	2.034	1.890
50	1.354	1.383	1.369	1.225
25	0.778	0.769	0.774	0.630
12.5	0.386	0.371	0.379	0.235
6.25	0.268	0.257	0.263	0.119
3.125	0.209	0.202	0.206	0.062
0	0.144	0.143	0.144

Materials provided

A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-mouse PF4 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled PF4 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
An instruction manual

Materials not provided

A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
50-300ul multi-channel micropipette
Pipette tips
Single-channel micropipette with different ranges
100ml and 500ml graduated cylinders
Deionized or distilled water
Timer
Test tubes for dilution

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Mouse PF4 ELISA Kit was designed for the quantitative measurement of Mouse PF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.125 ng/mL-200 ng/mL and the sensitivity is 1.603 ng/mL.

Target Background

Function
(From Uniprot)

Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation.

Gene References into Functions

these data demonstrate that PF4 has an important role in increasing B cell differentiation in the bone marrow environment PMID: 28914425
these results identify CXCL4 as a vital immunoregulatory chemokine essential for protecting mice against influenza A virus infection, especially as it affects the development of lung injury and neutrophil mobilization to the inflamed lung PMID: 28120850
These results indicate that CXCL4 is a novel Ni-binding protein that augments Ni allergy at the elicitation and sensitization phases. This is the first study to demonstrate that the Ni-binding protein augments Ni allergy in vivo. PMID: 28319310
PF4 was produced by Ly6G+CD11b+ immature myeloid cells in the early stage premetastatic lungs, and decreased during metastatic progression. PMID: 27223426
These data demonstrate that the CXCR2 network and CXCL4 play a role in the maintenance of normal HSC/HPC cell fates, including survival and self-renewal. PMID: 27222476
Platelet-derived CXCL7 and CXCL4 contribute to the pathogenesis of acute lung injury. PMID: 27755915
CXCL4 plays an important role in pancreatic inflammation PMID: 27183218
Platelet secretion of CXCL4 is Rac1-dependent and regulates neutrophil infiltration and tissue damage in septic lung damage PMID: 26478565
PF4 has a complex intramedullary life cycle with important implications in megakaryopoiesis and hematopoietic stem cell replication not seen with other tested alpha granule proteins. PMID: 26256688
Heparin enhances antigen uptake and activation of the initial steps in the cellular immune response to PF4-containing complexes. PMID: 25960020
Data indicate that platelet factor 4 (PF4) is involved directly in liver innate immune response against ischaemia-reperfusion injury (IRI) by regulating Th17 cell differentiation. PMID: 25440775
CXCL4 regulates hematopoietic stem cell cell cycle activity. PMID: 25326802
Platelet factor 4 has a role in regulating Th17 differentiation and cardiac allograft rejection PMID: 24463452
Histones regulate activated protein C formation in a manner similar to PF4 and suggest heparinoids may be benificial in sepsis. PMID: 24177324
PF4 drives a vascular smooth muscle inflammatory phenotype including a decline in differentiation markers, increased cytokine production, and cell proliferation. PMID: 23568488
PF4 expression on intestinal epithelial cells is increased after IR at both the mRNA and protein levels. In conclusion, these findings demonstrate that may PF4 represent an important mediator of local and remote tissue damage. PMID: 22792197
These results suggest that Cxcl4 and Cxcl7 play a role in the development of neurobehavioral alterations that are triggered by in utero TCDD exposure and later surface in adults. PMID: 21509788
PF4 may have a role in bacterial defense, and heparin-induced thrombocytopenia is probably a misdirected antibacterial host defense mechanism. PMID: 20959601
In vivo, half-life and diffusibility of CXCL4L1 compared with Cxcl4 is strongly increased. PMID: 20688960
These data indicate that PF4 induction of monocyte KLF4 expression may be an important step in the pathogenesis of experimental cerebral malaria. PMID: 20454664
Micronutrients effectively promote tumor dormancy in early prostate cancer by inducing platelet factor-4 expression and concentrating it at the tumor endothelium through enhanced platelet binding. PMID: 20525356
CXC chemokine ligand 4 (Cxcl4) is a platelet-derived mediator of experimental liver fibrosis. PMID: 20162727
important role for PF4 in thrombosis and neutralization of PF4 is an important component of heparin's anticoagulant effect. PMID: 14764524
Platelet factor 4 and interleukin-8 CXC chemokine heterodimer formation modulates function at the quaternary structural level PMID: 15531763
the PF4 storage pathway in alpha-granules is not a default pathway, but rather a regular granule storage pathway probably requiring specific sorting mechanisms PMID: 15613031
analysis of granule targeting sequence within platelet factor 4 PMID: 15964840
clinical heterogeneity in the HIT immune response may be due in part to requirements for specific biophysical parameters of the PF4/heparin complexes that occur in settings of intense platelet activation and PF4 release PMID: 17848616
platelet-associated PF-4, but not its plasma counterpart, may represent a potential biomarker of early tumor presence PMID: 17914028
platelet specific chemokine PF4 promotes atherosclerotic lesion development in vivo PMID: 18000617
Brain microglia are a cellular source of CXCL4 gene expression. PMID: 18248618
platelet-derived PF4 contributes to immune activation and T cell trafficking as part of the pathogenesis of experimental cerebral malaria . PMID: 18692777
Platelet factor 4 regulates megakaryopoiesis through low-density lipoprotein receptor-related protein 1 (LRP1) on megakaryocytes. PMID: 19605848

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Subcellular Location

Secreted.

Protein Families

Intercrine alpha (chemokine CxC) family

Database Links

KEGG: mmu:56744

STRING: 10090.ENSMUSP00000031320

UniGene: Mm.332490

Code	CSB-E07884m
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
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Mouse Platelet Factor 4,PF-4 ELISA Kit

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