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Product Name:

Rat Caspase 3,Casp-3 ELISA Kit

Product Type : ELISA Kit
Code : CSB-E08857r
Size : 96T,5×96T,10×96T
Uniprot NO. : P55213
Abbreviation : CASP3
Protein Biological Process 1 : Apoptosis/Autophagy
Alias : CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3
Species : Rat
Protein Biological Process 3 : Apoptosis
Sample Types : serum, plasma, tissue homogenates
Detect Range : 0.312 ng/ml-20 ng/ml
Sensitivity : 0.078 ng/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
HGNC : 1504
RGD : 2275
MGI : 107739
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat Casp-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %84
Range %80-92
1:2Average %98
Range %91-105
1:4Average %99
Range %92-110
1:8Average %91
Range %86-98
Recovery :
The recovery of rat Casp-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9690-100
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
202.915 2.903 2.909 2.828
102.321 2.283 2.302 2.221
51.467 1.444 1.456 1.375
2.50.727 0.706 0.717 0.636
1.250.365 0.359 0.362 0.281
0.6250.216 0.206 0.211 0.130
0.3120.137 0.129 0.133 0.052
00.084 0.078 0.081  
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