COPB2 Antibody

Datasheet

Citations (2)

Code	CSB-PA529993ESR1HU
Size	US$166
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Image	Western blot All lanes: COPB2 antibody at 2.51μg/ml Lane 1: Human placenta tissue Lane 2: MCF-7 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HepG2 whole cell lysate Lane 5: Jurkat whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 103, 100 kDa Observed band size: 103 kDa Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA529993ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA529993ESR1HU at dilution of 1:100
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) COPB2 Polyclonal antibody

Uniprot No.

P35606

Target Names

COPB2

Alternative Names

Beta'-coat protein antibody; Beta'-COP antibody; Coatomer subunit beta' antibody; Copb2 antibody; COPB2_HUMAN antibody; p102 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Coatomer subunit beta\\\' protein (657-906AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the COPB2 polyclonal antibody, a rabbit was immunized with the recombinant human COPB2 protein (657-906aa), followed by the collection of the rabbit anti-serum. The COPB2 antibody was purified through antigen affinity chromatography.

The rabbit anti-human COPB2 polyclonal antibody's specificity was verified by ELISA, WB, and IHC applications.It only reacts with human COPB2 protein. The COPB2 target protein plays a specific role in the formation of COPI-coated vesicles, which are responsible for transporting cargo retrogradely from the Golgi to the endoplasmic reticulum (ER).

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Identification of the Wnt signal peptide that directs secretion on extracellular vesicles U Gurriaran-Rodriguez, D Datzkiw, LG Radusky,Science Advances,2024
Wnt binding to Coatomer proteins directs secretion on exosomes independently of palmitoylation U Gurriaran-Rodriguez,bioRxiv,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Western blot

Sample type: cell

Review: knock down of COPB2 resulted in an abrogation of Wnt7a secretion on EVs.

By Anonymous

Target Background

Function

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.; This coatomer complex protein, essential for Golgi budding and vesicular trafficking, is a selective binding protein (RACK) for protein kinase C, epsilon type. It binds to Golgi membranes in a GTP-dependent manner.

Gene References into Functions

The increased expression of COPB2 was significantly correlated with the progression of lung adenocarcinoma. PMID: 29674272
High expression of COPB2 was found in prostate cancer tissue and was related to poor overall survival based on a public dataset. Cell proliferation was significantly inhibited in COPB2-knockdown CWR22RV1 cells, as demonstrated by CCK8 and colony formation assays. PMID: 29129687
COPB2 significantly promoted PC-3 cell proliferation and colony formation through the cell cycle and apoptosis pathway. COPB2 showed a clinical correlation and may serve as a biomarker for the detection for prostate cancer. PMID: 27986120
these data suggest that beta-COP plays a critical role in the forward transport of TREK1 channel to the plasma membrane. PMID: 20362547

Subcellular Location

Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.

Protein Families

WD repeat COPB2 family

Database Links

HGNC: 2232

OMIM: 606990

KEGG: hsa:9276

STRING: 9606.ENSP00000329419

UniGene: Hs.681616

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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