INVS Antibody

Datasheet

Code	CSB-PA011769LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: JK whole cell lysate(30µg), SY5Y whole cell lysate(30µg), HL-60 whole cell lysate(30µg), THP-1 whole cell lysate(30µg) All lanes: INVS antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 118,100 kDa Observed band size: 118 kDa Exposure time: 120s Immunofluorescence staining of Hela cell with CSB-PA011769LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) INVS Polyclonal antibody

Uniprot No.

Q9Y283

Target Names

INVS

Alternative Names

INVS antibody; INV antibody; NPHP2 antibody; Inversin antibody; Inversion of embryo turning homolog antibody; Nephrocystin-2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from INVS

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB,IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Required for normal renal development and establishment of left-right axis. Probably acts as a molecular switch between different Wnt signaling pathways. Inhibits the canonical Wnt pathway by targeting cytoplasmic disheveled (DVL1) for degradation by the ubiquitin-proteasome. This suggests that it is required in renal development to oppose the repression of terminal differentiation of tubular epithelial cells by Wnt signaling. Involved in the organization of apical junctions in kidney cells together with NPHP1, NPHP4 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis.

Gene References into Functions

Collectively, these results indicated that inversin might promote the tumorigenicity of lung cancer cells and serve as a novel therapeutic target of non-small cell lung cancer. PMID: 28618971
Akt phosphorylates inversin at amino acids 864-866 that are required not only for Akt interaction, but also for inversin dimerization. PMID: 27220846
Truncating mutations in NPHP2 gene is associated with nephronophthisis-related ciliopathy. PMID: 23559409
INVS mutation can cause juvenile nephronophthisis with abnormal reactivity of the Wnt/beta-catenin pathway. PMID: 20798123
has conserved ankyrin repeat and IQ domains and interacts with calmodulin PMID: 11941489
analysis of a homozygous mutation in exon 13 of inversin (INVS) (C2719T, R907X) in a patient with retinitis pigmentosa and renal failure [case report] PMID: 16522655
Thus, although considered a common variant, inv(10)(p11.2q21.2) has a single ancestral founder among northern Europeans. PMID: 16642442
new mutant genes might be responsible for the early onset of ESRD in infantile NPHP with features of JBTS PMID: 17216245
Cystic kidney disease has been linked to mutations in the Invs gene in mice with an inversion of embryonic turning (inv/inv) and the INVS (NPHP2) gene in human infantile nephronophthisis (NPH). PMID: 18218308

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Involvement in disease

Nephronophthisis 2 (NPHP2)

Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Membrane; Peripheral membrane protein. Nucleus. Cell projection, cilium. Note=Associates with several components of the cytoskeleton including ciliary, random and polarized microtubules. During mitosis, it is recruited to mitotic spindle. Frequently membrane-associated, membrane localization is dependent upon cell-cell contacts and is redistributed when cell adhesion is disrupted after incubation of the cell monolayer with low-calcium/EGTA medium.

Tissue Specificity

Widely expressed. Strongly expressed in the primary cilia of renal tubular cells.

Database Links

HGNC: 17870

OMIM: 243305

KEGG: hsa:27130

STRING: 9606.ENSP00000262457

UniGene: Hs.558477

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Wnt signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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