RAB12 Antibody

Code CSB-PA753710LA01HU
Size US$166
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Image
  • Western blot
    All lanes: RAB12 antibody at 2μg/ml + Hela whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 28 kDa
    Observed band size: 28, 36 kDa

  • Immunofluorescent analysis of Hela cells using CSB-PA753710LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RAB12 Polyclonal antibody
Uniprot No.
Target Names
RAB12
Alternative Names
Putative Ras related protein Rab 12 antibody; RAB12 antibody; RAB12; member RAS oncogene family antibody; RAB12_HUMAN antibody; Ras-related protein Rab-12 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Ras-related protein Rab-12 protein (98-241AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RAB12 Antibody (Product code: CSB-PA753710LA01HU) is Non-conjugated. For RAB12 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA753710LB01HU RAB12 Antibody, HRP conjugated ELISA
FITC CSB-PA753710LC01HU RAB12 Antibody, FITC conjugated
Biotin CSB-PA753710LD01HU RAB12 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may play a role in protein transport from recycling endosomes to lysosomes regulating, for instance, the degradation of the transferrin receptor. Involved in autophagy.
Gene References into Functions
  1. DENND3 is the exchange factor for the small GTPase Rab12 regulated through an intramolecular interaction PMID: 28249939
  2. a novel signaling pathway identified whereby starvation-induced activation of ULK leads to phosphorylation of endogenous DENND3, with subsequent activation of Rab12 and initiation of membrane trafficking events required for autophagy PMID: 25925668
  3. findings demonstrate the functional importance of Rab12 for retrograde toxin trafficking PMID: 24703428
Subcellular Location
Recycling endosome membrane; Lipid-anchor; Cytoplasmic side. Lysosome membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome.
Protein Families
Small GTPase superfamily, Rab family
Database Links

HGNC: 31332

OMIM: 616448

KEGG: hsa:201475

STRING: 9606.ENSP00000331748

UniGene: Hs.270074

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