THOC1 Antibody

Datasheet

Code	CSB-PA853419ESR1HU
Size	US$166
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Image	Western blot All lanes: THOC1 antibody at 3μg/ml Lane 1: Jurkats whole cell lysate Lane 2: 293T whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 76, 44 kDa Observed band size: 76 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA853419ESR1HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human breast cancer using CSB-PA853419ESR1HU at dilution of 1:100 Chromatin Immunoprecipitation 293T (1.6*10⁶) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4μg anti-THOC1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified tissue using real-time PCR with primers (CSB-PP853419HU) against the RHOX5 promoter.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) THOC1 Polyclonal antibody

Uniprot No.

Q96FV9

Target Names

THOC1

Alternative Names

hTREX84 antibody; Death domain containing protein p84N5 antibody; HPR 1 antibody; HPR1 antibody; hTREX84 antibody; Nuclear matrix protein p84 antibody; P84 antibody; p84N5 antibody; Tho 1 antibody; THO complex 1 antibody; THO complex subunit 1 antibody; Tho1 antibody; THOC 1 antibody; Thoc1 antibody; THOC1_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human THO complex subunit 1 protein (428-657AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Form

Liquid

Tested Applications

ELISA, WB, IHC, ChIP

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Required for efficient export of polyadenylated RNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production. Regulates transcriptional elongation of a subset of genes. Involved in genome stability by preventing co-transcriptional R-loop formation.; Participates in an apoptotic pathway which is characterized by activation of caspase-6, increases in the expression of BAK1 and BCL2L1 and activation of NF-kappa-B. This pathway does not require p53/TP53, nor does the presence of p53/TP53 affect the efficiency of cell killing. Activates a G2/M cell cycle checkpoint prior to the onset of apoptosis. Apoptosis is inhibited by association with RB1.

Gene References into Functions

THO interacts with the Sin3A histone deacetylase complex to suppress co-transcriptional R-loops, DNA damage, and replication impairment. Functional analyses show that histone hypo-acetylation prevents accumulation of harmful R-loops and RNA-mediated genomic instability. PMID: 29074626
Colorectal cancer patients with high levels of Thoc1 expression had poorer overall-survival and disease-free survival, whereas those with lower levels of Thoc1 expression survived longer. PMID: 26545775
In humans, high THOC1 protein expression associates with prostate cancer aggressiveness and recurrence. Thus, THOC1 protein is a molecular marker that may improve the identification of aggressive prostate cancers, potentially reducing overtreatment. PMID: 25296641
Thoc1 inhibits cell growth via induction of cell cycle arrest and apoptosis in lung cancer cells. PMID: 24682263
This suggests NEDD4-1 functions in conjunction with other post-translational mechanisms to regulate Thoc1 protein and THO activity. PMID: 23460917
overexpression of hTREX84 is associated with cancer cell transformation, proliferation and may be regulated by RelA/p65 PMID: 22952718
We show that human THO depletion impairs transcription elongation and mRNA export and increases instability associated with DNA breaks, leading to hyper-recombination and gammaH2AX and 53BP1 foci accumulation PMID: 22144908
A differential connection between tumorogenesis and the expression levels of human THO and ALY. PMID: 21329510
hHpr1/p84/Thoc1 regulates transcriptional elongation and may participate in a protein complex functionally analogous to yeast TREX, physically linking elongating RNA polymerase II with RNA processing factors PMID: 15870275
recruitment of the human TREX complex to spliced mRNA is not directly coupled to transcription, but is instead coupled to transcription indirectly through splicing PMID: 15998806
Thoc1 may be important for neoplastic transformation PMID: 17638875
Cells respond to AAV infection by activating two DNA damage signaling cascades. The first activates the p84N5 protein, which in turn activates caspase-6, leading to cell death. PMID: 17699767
elevated expression of hHpr1/p84/Thoc1 is common in NSCLC and may have prognostic significance in subgroups of patients PMID: 18469354

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Subcellular Location

[Isoform 1]: Nucleus speckle. Nucleus, nucleoplasm. Nucleus matrix. Cytoplasm. Note=Can shuttle between the nucleus and cytoplasm. Nuclear localization is required for induction of apoptotic cell death. Translocates to the cytoplasm during the early phase of apoptosis execution.; [Isoform 2]: Cytoplasm.

Tissue Specificity

Ubiquitous. Expressed in various cancer cell lines. Expressed at very low levels in normal breast epithelial cells and highly expressed in breast tumors. Expression is strongly associated with an aggressive phenotype of breast tumors and expression correl

Database Links

HGNC: 19070

OMIM: 606930

KEGG: hsa:9984

STRING: 9606.ENSP00000261600

UniGene: Hs.712543

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