Sumo tag Monoclonal Antibody

Name: Sumo tag Monoclonal Antibody
Brand: CUSABIO
SKU: CSB-MA000131M0m
Price: 120 USD
Availability: InStock
Rating: 5.0 (1 reviews)

Datasheet

Citations (1)

Code	CSB-MA000131M0m
Size	US$120
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Image	All Lanes The cell supernatant hybridoma of Sumo tag Lane 1 Sumo tagged fusion protein at 20ng Lane 2 Sumo tagged fusion protein at 2ng Lane 3 Sumo tagged fusion protein at 0.2ng Lane 4 Sumo tagged fusion protein at 0.02ng Secondary Goat polyclonal to mouse IgG at 1/15000 dilution Predicted band size : 50kd Observed band size: 50kd Additional bands at : 25 kd
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Product Details
Citations
Customer Reviews and Q&A

Product Details

Raised in

mouse

Species Reactivity

N/A

Immunogen

sumo tag protein

Conjugate

Non-conjugated

Clonality

monoclonal

Isotype

IgG1

Clone No.

20G12

Purification Method

>95%,Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

liquid

Tested Applications

ELISA,WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SUMO (Small Ubiquitin-like Modifier) tag is a powerful fusion tag widely utilized in recombinant protein expression systems to significantly enhance protein solubility, stability, and overall yield. As an essential affinity tag, it plays a pivotal role in the efficient production and subsequent purification of recombinant proteins for structural and functional analysis. This mouse SUMO tag monoclonal antibody (IgG1) is generated against the native SUMO tag protein and purified via Protein G affinity chromatography, ensuring high purity (>95%). It demonstrates exceptional sensitivity and specificity in Western Blot and ELISA applications. As demonstrated in the validation data, the antibody reliably detects SUMO-tagged fusion proteins across a wide concentration range, from 20 ng down to an ultra-sensitive limit of 0.02 ng. The observed band size perfectly aligns with the predicted 50 kD molecular weight, providing accurate identification of your fusion constructs. As a high-affinity tag antibody, this reagent is an indispensable tool for researchers verifying recombinant protein expression, monitoring the efficiency of SUMO protease cleavage, and performing affinity purification. It provides a reliable quality control checkpoint in protein engineering workflows. Cited in peer-reviewed publications, this antibody offers a well-validated solution for your demanding molecular biology experiments.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Inhibition of Chikungunya virus early replication by intracellular nanoantibodies targeting nsP2 Epitope Rich Region Q Deng,Antiviral Research,2022

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : wb

Sample type: cells

Review: 100 μL of HRP-conjugated anti-SUMO tag antibody (1:2000, CUSABIO) was added to the well and incubated for 1 h.

By Anonymous

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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