Recombinant Human Nectin-2 (NECTIN2), partial (Active)

Nectin-2 (CD112) serves as a key ligand for the inhibitory receptor PVRIG, making it a critical target for studying immune checkpoint regulation in tumor microenvironments. This recombinant human Nectin-2 construct, spanning residues 32–360 of isoform alpha with a C-terminal 6xHis tag, demonstrates confirmed binding to both human PVRIG-mFc and PVRIG-Fc in functional ELISA, yielding ED50 values of 3.72 µg/ml and 2.914 µg/ml respectively — data that supports use in receptor-ligand binding assays, checkpoint blocking antibody screening, and PVRIG pathway characterization studies. Mammalian cell expression preserves native-like glycosylation patterns essential for physiologically relevant protein-protein interactions. With purity exceeding 95% by SDS-PAGE and endotoxin levels below 1.0 EU/µg by LAL testing, this preparation satisfies the quality criteria typical for functional cell-based assays investigating T-cell or NK-cell co-inhibitory signaling as well as ELISA-based assay development.