Product Details

Purity

>96% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 5.0-20 ng/ml.

Target Names

CCL4

Uniprot No.

P13236

Research Area

Immunology

Alternative Names

MIP 1 beta; Secreted protein G 26; ACT 2; ACT-2; ACT2; AT744.1; AT744.2; C C motif chemokine 4; C C motif chemokine 4 like; C C motif chemokine ligand 4 like 1; C C motif chemokine ligand 4 like 2; CC chemokine ligand 4; CC chemokine ligand 4L1; CC chemokine ligand 4L1d2; CC chemokine ligand 4L2; CCL4; CCL4_HUMAN; ccl4l 1; CCL4L; CCL4L1; Chemokine (C C motif) ligand 4; Chemokine (C C motif) ligand 4 like 1; Chemokine (C C motif) ligand 4 like 1; telomeric; Chemokine (C C motif) ligand 4 like 2; Chemokine CC Motif Ligand 4; G 26; G 26 T lymphocyte secreted protein; G-26 T-lymphocyte-secreted protein; HC21; Immune activation 2; LAG 1; LAG-1; LAG1; Lymphocyte activation gene 1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1 beta; Macrophage inflammatory protein 1-beta; Macrophage inflammatory protein 1b2; MGC104418; MGC126025; MGC126026; MIP-1-beta; MIP-1-beta(1-69); MIP-1-beta(3-69); MIP1 beta; MIP1B; MIP1B1; Monocyte adherence induced protein 5 alpha; PAT 744; Protein H400; SCYA2; SCYA4; SCYA4L; SCYA4L1; SCYA4L2; SCYQ4L2; Secreted protein G 26; Secreted protein G26; SIS gamma; SIS-gamma; Small inducible cytokine A4 (homologous to mouse Mip 1b); Small inducible cytokine A4; small inducible cytokine A4-like; Small-inducible cytokine A4; T cell activation protein 2; T-cell activation protein 2

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

24-92aa

Complete Sequence

APMGSDPPTA CCFSYTARKL PRNFVVDYYE TSSLCSQPAV VFQTKRSKQV CADPSESWVQ EYVYDLELN

Mol. Weight

7.8 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag-Free

Form

Lyophilized powder

Buffer

0.2 μm filtered 20 mM PB, pH 7.4, 150 mM NaCl ,lyophilized

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CCL4 (MIP-1β) is a key CC chemokine that drives monocyte and T-cell recruitment through CCR5 signaling, making it central to studies of inflammatory trafficking and HIV co-receptor biology. This tag-free recombinant human CCL4, encompassing the full-length mature sequence (residues 24–92), demonstrates potent chemotactic activity on human peripheral blood monocytes at 5–20 ng/ml, providing a suitable basis for Transwell migration assays, CCR5-dependent signaling studies involving MAPK and NF-κB pathway activation, and in vivo inflammation models. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that can confound monocyte and macrophage functional readouts, while purity exceeding 96% by SDS-PAGE supports use as a reliable reference standard in chemokine detection assays or as a coating antigen for antibody validation by ELISA. The E. coli-derived, untagged format faithfully represents the native mature protein, aligning with standards expected in receptor-binding and cell-based activation experiments.

Target Background

Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Gene References into Functions

identified 13 ADCC-activated genes. Six gene expression assays including 8 of the 13 genes (CCL3, CCL4/CCL4L1/CCL4L2, CD160, IFNG, NR4A3 and XCL1/XCL2) were analyzed in 127 kidney biopsies PMID: 25449536
PCR results show that lower copy numbers of CCL4L1 are observed in the Caucasian population nd higher copy numbers are observed in the Black population of South Africa. PMID: 24727646
CCL4L1 allele frequency is higher in severe psoriasis, whereas CCL4L2 is more frequent in patients with a milder disease. PMID: 21614014
multiple copies of CCL3L1 and CCL4L1 present in a single diploid genome are the result of segmental duplication at chromosome 17q12 PMID: 15028295

Subcellular Location

Secreted.

Protein Families

Intercrine beta (chemokine CC) family

Database Links

HGNC: 10630

OMIM: 182284

KEGG: hsa:388372

STRING: 9606.ENSP00000250151

UniGene: Hs.449862

Code	CSB-AP000861HU
Abbreviation	Recombinant Human CCL4 protein (Active)
MSDS	MSDS Datasheet
Size	$354
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Recombinant Human C-C motif chemokine 4 protein (CCL4) (Active)

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