Product Details

Purity

>98% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/μg as determined by LAL method.

Activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human T-lymphocytes is in a concentration of 10-100 ng/ml.

Target Names

XCL1

Uniprot No.

P47992

Research Area

Immunology

Alternative Names

ATAC; C motif chemokine 1; Chemokine (C motif) ligand 1; Chemokine C Motif Ligand 1; Cytokine SCM-1; LPTN; LTN; Lymphotactin; Lymphotaxin; SCM 1 alpha; SCM 1; SCM 1a; SCM-1-alpha; SCM1; SCM1A; SCYC1; Single cysteine motif 1a; Small inducible cytokine C1; Small Inducible Cytokine Subfamily C Member 1; Small inducible cytokine subfamily C, member 1 (lymphotactin); Small-inducible cytokine C1; X-C motif chemokine ligand 1; XC chemokine ligand 1; Xcl1; XCL1_HUMAN

Species

Homo sapiens (Human)

Source

E.Coli

Expression Region

23-114aa

Complete Sequence

GSEVSDKRTC VSLTTQRLPV SRIKTYTITE GSLRAVIFIT KRGLKVCADP QATWVRDVVR SMDRKSNTRN NMIQTKPTGT QQSTNTAVTL TG

Mol. Weight

10.2 kDa

Protein Length

Partial

Tag Info

Tag-Free

Form

Liquid or Lyophilized powder

Buffer

Lyophilized from a 0.2 µm filtered 20 mM PB, 150mM NaCl, pH 7.4

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

5-10 business days

Datasheet & COA

Please contact us to get it.

Description

Lymphotactin (XCL1) is the sole C-class chemokine in humans, signaling exclusively through XCR1 to recruit T cells and dendritic cell subsets—making it a critical tool for dissecting XCR1-mediated immune crosstalk. This tag-free recombinant protein spans residues 23–114, corresponding to the mature secreted sequence, and drives chemotaxis of primary human T-lymphocytes at 10–100 ng/ml, providing a suitable basis for Transwell migration assays, XCR1 receptor internalization studies, and downstream MAPK or calcium flux signaling experiments. Endotoxin levels below 1.0 EU/μg minimize LPS-driven artifacts that commonly confound immune cell functional readouts, supporting use in sensitive in vivo inflammation models and primary cell activation assays where cytokine-specific effects must be cleanly resolved. Purity exceeding 98% by SDS-PAGE further qualifies this protein as a reliable reference standard in XCL1 detection ELISAs and as a positive control for antibody validation in Western blot.

Target Background

Function

Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.

Gene References into Functions

higher serum XCL1 levels at diagnosis and their progressive decline throughout chemotherapy could be correlated with higher survival. PMID: 29027574
This study defines a Glycosaminoglycan binding surface on XCL1 dimer that includes residues that are important for HIV-1 inhibition. PMID: 26836755
Electrostatics and Hydrophobic Effects in the Metamorphic Protein Human Lymphotactin PMID: 26134347
XCL1 acts via direct interaction with the external viral envelope glycoprotein, gp120, to block HIV-1 infection PMID: 26085164
analysis of XCL1 and XCL2, members of the C-chemokine subfamily, in the immune system PMID: 25497737
XCL1 displays antimicrobial activity against E. coli and S. aureus. PMID: 12949249
XCL1-mediated inhibition is associated with direct interaction of the chemokine with the HIV-1 envelope. PMID: 24385911
In native annulus fibrosus tissue homeostasis and repair, CXCR3 is evident, whereas XCL1 could not be detected. PMID: 21270681
The expression patterns of XCR1 and XCL1 were conserved in human and mice blood cells, including certain dendritic cell subsets. PMID: 20541533
Data indicated increased expression of XCL1 in CD4+ and CD8+ T cells in Wegener's granulomatosis (WG), and suggest that XCL1 might be a key modulator of T cell recruitment in WG. PMID: 19797511
NMR spectra of human lymphotactin (hLtn), obtained under various solution conditions, have revealed that the protein undergoes a major conformational rearrangement dependent on temperature and salt concentration. PMID: 11889129
lymphotactin, as well as MCP-1, may contribute to leukocyte infiltration and disease progression in IgA nephropathy. PMID: 12053063
Lptn might be a key modulator for T cell trafficking in the pathogenesis of rheumatoid arthritis PMID: 12847680
In humans XCL1 expression is mainly a property of CD8+ T cells; the contribution to its synthesis by different T cell receptor alpha beta-expressing T cell subsets, namely CD4+ lymphocytes, is negligible. PMID: 14568926
lymphotactin utilizes highly specific glycosaminoglycan-binding sites PMID: 14707146
XCL1 activation is positively correlated with HIV-1 Tat expression in human U87.MG astrocytes in vitro. PMID: 14734774
description of a lymphotactin-producing monoclonal T-cell lymphoproliferative disorder with extreme lymphocytopenia and progressive leukoencephalopathy [case report] PMID: 16923584
The monocyte-derived dendritic cells can express Lptn mRNA in a maturation-dependent manner. PMID: 17077010
Elevated serum lymphotactin levels correlate with relatively milder manifestations in diffuse cutaneous systemic sclerosis (dSSc), especially lower severity of lung involvement, suggesting that lymphotactin may play a role in the development of dSSc. PMID: 18322986
functional repertoire and regulation of a single naturally occurring amino acid sequence can be expanded by access to a set of highly dissimilar native-state structures PMID: 18364395
XCL1 enhances regulatory activities of CD4+ CD25(high) CD127(low/-) T cells in human allergic asthma PMID: 18832695
ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein PMID: 18838386

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Subcellular Location

Secreted.

Protein Families

Intercrine gamma family

Tissue Specificity

Highest level in spleen, lower in peripheral leukocytes and very low levels in lung, colon and small intestine.

Database Links

HGNC: 10645

OMIM: 600250

KEGG: hsa:6375

STRING: 9606.ENSP00000356792

UniGene: Hs.458346

Code	CSB-AP000791HU
Abbreviation	Recombinant Human XCL1 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$142
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Recombinant Human Lymphotactin protein (XCL1), partial (Active)

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