Recombinant Rat Growth-regulated alpha protein(Cxcl1) (Active)

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Code CSB-AP001391RA
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Product Details

Purity >97% as determined by SDS-PAGE and HPLC.
Endotoxin Less than 1.0 EU/μg as determined by LAL method.
Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.
Target Names Cxcl1
Uniprot No. P14095
Research Area Immunology
Alternative Names Cxcl1; Cinc1; Gro; Scyb1Growth-regulated alpha protein; C-X-C motif chemokine 1; Cytokine-induced neutrophil chemoattractant 1; CINC-1; Platelet-derived growth factor-inducible protein KC
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 25-96aa
Complete Sequence APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREA CLDPEAPMVQ KIVQKMLKGV PK
Mol. Weight 7.8 kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Lyophilized powder
Buffer Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 5-10 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
Gene References into Functions
  1. The results showed that after short-term incubation both CXCL1 and CXCL2 can activate TRPV1+/IB4+ DRG neurons by significantly reducing TRPV1 desensitization, while at acute application both chemokines induced an increase in [Ca2+]i although of different amplitudes, in a subset of neurons partially also itch-sensitive in the case of CXCL1, or non-itch sensitive in the case of CXCL2. PMID: 28820395
  2. remote ischemic preconditioning protects hepatic microcirculation by induction of HO and modulation of cytokine induced neutrophil chemoattractant in hepatic ischemia reperfusion injury PMID: 27672274
  3. Results results demonstrate that sphingosine-1-phosphate induces Ca(2+) signaling in astrocytes via Gq -coupled receptors S1P2 and S1P3 , followed by Ca(2+) influx through TRPC6 that could activate MAPK signaling, which leads to increased secretion of the proinflammatory or neuroprotective chemokine CXCL1 PMID: 28300348
  4. aging can reduce the expression of CINC and MCP-1 mRNA in lung tissues, and reduce the infiltration of neutrophils and monocyte-macrophages induced by CINC and MCP-1. This might lead to increased risk of pneumonia in elderly patients. PMID: 24464584
  5. In opioid-tolerant rats, CXCL1 is up-regulated in CSF; as it is in patients also. PMID: 25383568
  6. Function of the transient receptor potential vanilloid (TRPV)1 channel is enhanced by dorsal root ganglia inflammation. The effects (decreased tachyphylaxis) are mimicked by incubation with GRO/KC. PMID: 22466126
  7. CXCL1 inhibition of oligodendrocyte progenitor cell migration is regulated via changes in intracellular calcium flux. PMID: 22554866
  8. hyperoxia exposure led to a suppression of the HDAC1/HDAC2 expression and activity, and the overall HDAC activity, as well as arrest of alveolarization, and an elevated expression of CINC-1 in the lungs of newborn rats PMID: 21905265
  9. Advanced glycation end products promote the secretion of MCP-1 and IL-8 in vascular smooth muscle cells. PMID: 21418993
  10. Severe burn injury activates pulmonary NF-kappaB and leads to secretion of IL8 and TNF alpha in the lung. PMID: 17959522
  11. In rats with chronic nonbacterial prostatitis, the levels of TNF-alpha and IL-8 in the serum and prostate tissues were significantly lower in the curcumin-treated group than in the positive control group. PMID: 20180411
  12. ATP-stimulated C6 glioma cells showed enhanced expression of MCP-1 & IL-8; elevated levels of MCP-1 & IL-8 are predicted to enhance mobility of tumor cells & promote recruitment of microglia into developing tumors thereby supporting tumor growth. PMID: 20003523
  13. These results suggest that IL-6 and CINC-1 contribute to alpha2M production in rats only when IL-6 and CINC-1 act synergistically. PMID: 21030786
  14. The decreased serum concentration of CINC-1 as neuroprotective agent and increased CINC-2alpha in late stage of Morris hepatoma may be considered for their contribution to cerebellar degeneration. PMID: 20602290
  15. Rats treated with fullerenes showed a transient significant increase of neutrophils and expression of CINC-1,-2alphabeta and -3. PMID: 20226088
  16. CINC-1, released at sites of inflammation, mediates inflammatory hyperalgesia in rats via release of sympathomimetic amines. PMID: 12517731
  17. CINC-1 also functions as an early acute-phase protein after injury to the brain or to peripheral tissues PMID: 12709409
  18. neutrophil migration induced by IL-8 is dependent on CINC-1 release from mast cells PMID: 12824006
  19. Data show that TII alveolar epithelial cells produce three of the major proinflammatory CXC chemokines (GRO, CINC-2alpha, and MIP-2) and their cognate receptor CXCR2. PMID: 12829448
  20. CINC, in contrast to MIP-2, is selectively transported from the lung to the systemic circulation, where it promotes neutrophil migration into the lung in response to a chemotactic stimulus. PMID: 14617513
  21. reactive oxygen species and lipid peroxidation mainly derived from neutrophils, which are stimulated and mobilized by TNF-alpha and cytokine-induced neutrophil chemoattractant-1 PMID: 15942680
  22. CINC-1 may contribute to liver repair and regeneration PMID: 16271365
  23. In summary, hyperalgesia, local PGE2 production, and spinal c-Fos expression occur after CFA-induced inflammation but not after CXCL1- or CXCL2/3-induced, selective PMN recruitment. PMID: 16522746
  24. results demonstrate that ascorbic acid deficiency elevates the serum, liver and spleen concentrations of cytokine-induced neutrophil chemoattractant-1 (CINC-1 as seen in acute inflammation PMID: 16637227
  25. Following cecal ligation and puncture CINC-1 production increases in the lung, setting the stage for neutrophil accumulation in lung during sepsis. PMID: 16818791
  26. activation of PAR-2, as well as PAR-1, can rescue astrocytes from ceramide-induced apoptosis via regulating chemokine GRO/CINC-1 release. PMID: 16942465
  27. berberine dose-dependently inhibited the expression of CINC-1 induced by LPS and diminished the anterior uveitis. PMID: 17164575
  28. TNFalpha has in different forms of arthritis, but points out the idea that CINC-1 (the homologue for human IL-8) may constitute a promising target for reactive arthritis management PMID: 17166735
  29. basal level of calcium is the pre-requisite for GRO/CINC-1 protein synthesis and secretion PMID: 17666044
  30. Induction of CXCL1 by extracellular matrix and autocrine enhancement by interleukin-1 in rat pancreatic beta-cells. PMID: 17702850
  31. GGA effectively suppressed the up-regulation of GRO1 messenger ribonucleic acid, and this was validated by Northern hybridization. PMID: 18683011
  32. This is the first time that centrally injected CINC-1 has been reported to act directly on the pyrogen-sensitive neurons of the preoptic area, promoting a thermoregulatory response that seems to depend on other endogenous pyrogens synthesis and PGE2. PMID: 18694739
  33. CXCL1 incubation (1.5 nM, overnight) caused marked upregulation of Na+ currents in acutely isolated small diameter rat (adult) sensory neurons in vitro. PMID: 18816377
  34. The results suggest that GRO/KC has important effects in inflammatory processes via its direct actions on sensory neurons. PMID: 19476648
  35. CINC-1 is a neutrophil chemoattractant; findings have established that CINC-1 also functions as an early acute-phase protein after injury to the brain or to peripheral tissues. PMID: 12709409

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Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Tissue Specificity At least expressed in the lung and trachea.
Database Links

KEGG: rno:81503

STRING: 10116.ENSRNOP00000003778

UniGene: Rn.10907

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