Ang4 Antibody

Code CSB-PA661010ZA01MO
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: Ang4 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 31.4 kDa
    Observed band size: 33 kDa
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Product Details

Uniprot No.
Target Names
Ang4
Alternative Names
Angiogenin-4 (EC 3.1.27.-), Ang4
Species Reactivity
Mus musculus (Mouse)
Immunogen
Recombinant Mus musculus (Mouse) Angiogenin-4 (25-144aa)
Immunogen Species
Mus musculus (Mouse)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The Ang4 polyclonal antibody is created by immunizing a rabbit with the recombinant Mus musculus (mouse) Angiogenin-4 (25-144aa). After collection from the rabbit serum, the Ang4 antibody is purified using protein G. This Ang4 antibody is specifically designed for detecting the Mus musculus (mouse) Ang4 protein in ELISA and WB experiments.

Ang4 belongs to the angiopoietin family of proteins, which play crucial roles in angiogenesis and vascular remodeling. Ang4 specifically influences blood vessel permeability and stability. It is involved in maintaining the integrity of blood vessels and controlling the movement of fluids and molecules across blood vessel walls. Additionally, Ang4 has been implicated in various physiological and pathological processes, including inflammation, wound healing, and tumor angiogenesis.

Customer Reviews and Q&A

 Customer Reviews
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Applications : Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sample type: Mouse colon epithelial tissues

Sample dilution: For WB, I use 1:1000 dilution, For IF, I use 1:500 dilution

Review: This antibody is really good for IF on frozen sections of mouse colon. Also, I got great target band by western blot (although there are some non-specific bands but at target size I see very clean band).

By Sanlan Li

Target Background

Function
Has bactericidal activity against E.faecalis and L.monocytogenes, but not against L.innocua and E.coli. Promotes angiogenesis (in vitro). Has low ribonuclease activity (in vitro). Promotes proliferation of melanoma cells, but not of endothelial cells or fibroblasts (in vitro).
Gene References into Functions
  1. Intestinal intraepithelial lymphocyte-enterocyte crosstalk regulates production of bactericidal angiogenin 4 by Paneth cells upon microbial challenge. PMID: 24358364
  2. The cellular source of mouse Ang4 in the colon following T. muris infection is the goblet cell and expression is under the control of IL-13. PMID: 22970115
  3. Expression of Reg3 proteins and Ang4 by goblet cells point to an important role for goblet cells in innate defense. PMID: 22723890
  4. differences in gene expression profiles associated with immune and defence responses, including angiogenin 4 PMID: 19852835
  5. protein structure of angiogenin 4 PMID: 15772759

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Subcellular Location
Cytoplasmic vesicle, secretory vesicle lumen. Secreted. Nucleus, nucleolus.
Protein Families
Pancreatic ribonuclease family
Tissue Specificity
Detected in small intestine, caecum and colon, with the highest expression in Paneth cells in the intestinal epithelium.
Database Links
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