CUL1 Antibody

Code CSB-PA822004XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CUL1 Polyclonal antibody
Uniprot No.
Target Names
CUL1
Alternative Names
CUL1 antibody; At4g02570 antibody; T10P11.26Cullin-1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana CUL1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in ubiquitination and subsequent proteasomal degradation of target proteins. Regulator of mitotic processes which plays a role during gametogenesis and embryogenesis. Together with SKP1, RBX1 and a F-box protein, it forms a SCF complex. The functional specificity of this complex depends of the type of F-box protein. SCF(UFO) is implicated in floral organ development. SCF(TIR1) is involved in auxin signaling pathway. SCF(COI1) regulates responses to jasmonates. SCF(EID1) and SCF(AFR) are implicated in phytochrome A light signaling. SCF(ADO1/ZTL), SCF(ADO2/LKP2), SCF(ADO3/FKF1) are related to the circadian clock. SCF(ORE9) seems to be involved in senescence. SCF(EBF1/EBF2) may regulate ethylene signaling.
Gene References into Functions
  1. ALF4 binds to RBX1 and inhibits the activity of SCF(TIR)(1), an E3 ligase responsible for degradation of the Aux/IAA transcriptional repressors. In vivo, the alf4 mutation destabilizes the CUL1 subunit of the SCF PMID: 29233834
  2. The axr6-101 phenotype is caused by the E716K substitution of the CUL1 protein, which is likely to affect its ability to bind to the C-terminal RING domain of RING-box 1 (RBX1). PMID: 26339842
  3. a new viable recessive allele of the Arabidopsis CULLIN1 gene in the non-reference Wassilewskija (Ws-4) accession, was identified. PMID: 24955772
  4. icu13 is a novel recessive allele of AUXIN RESISTANT6 (AXR6), which encodes CULLIN1, an invariable component of the SCF complex.[ICU13] [ncurvata13] PMID: 23319550
  5. Genetic and physiological data to directly demonstrate that AtCUL1 is necessary for normal JA responses are presented. PMID: 15860010
  6. A viable and fertile weak allele of CUL1, called cul1-6, is described. PMID: 17158585
  7. CUL1 is required for TOC1 degradation and suggests that this protein is the functional cullin circadian clock. PMID: 18433436
  8. the first reported allele of CUL1 to directly affect subunit interactions at the CUL1 C terminus PMID: 19114460
  9. CUL1-based SCF E3 ligase activity is required for Della protein degradation. PMID: 19717618

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Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, phragmoplast. Note=Mainly nuclear during interphase and preprophase, but also weakly cytoplasmic during interphase. Associated to mitotic spindle during metaphase, and to the phragmoplast during telophase.
Protein Families
Cullin family
Tissue Specificity
Expressed constitutively in roots, seedlings, stems, leaves and flowers.
Database Links

KEGG: ath:AT4G02570

STRING: 3702.AT4G02570.1

UniGene: At.24877

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