E3L Antibody

Code CSB-PA322729ZA01VAA
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: E3L antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 28.5 kDa
    Observed band size: 29 kDa
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Product Details

Full Product Name
Rabbit anti-Vaccinia virus (strain Copenhagen) (VACV) E3L Polyclonal antibody
Uniprot No.
Target Names
E3L
Alternative Names
E3L antibody; Protein E3 antibody; p25 antibody
Raised in
Rabbit
Species Reactivity
Vaccinia virus
Immunogen
Recombinant Vaccinia virus Protein E3 (E3L) (1-190AA)
Immunogen Species
Vaccinia virus (strain Copenhagen) (VACV)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The development of the E3L polyclonal antibody follows a well-defined procedure. It initiates with the repeated immunization of a rabbit using recombinant vaccinia virus E3 protein (1-190aa) until an optimal antibody titer is reached. Subsequently, the rabbit's blood is collected, and the antibodies are purified from the serum using protein A/G. The functionality of the resulting E3L antibody is thoroughly assessed through ELISA and WB applications, confirming its specific reactivity with the vaccinia virus E3 protein.

The E3L gene encodes the vaccinia virus E3 protein, a double-stranded RNA (dsRNA)-binding protein that inhibits the activation of interferon (IFN)-induced dsRNA-dependent protein kinase (PKR) and acts as an inhibitor of the IFN-induced 2-5A-synthetase enzyme. In addition to IFN-induced antiviral activity, E3 is also a key host-range protein, which suppresses the innate antiviral immune response of the infected cells.

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Target Background

Function
Plays a role in the inhibition of multiple cellular antiviral responses activated by dsRNA, such as inhibition of PKR activation, apoptosis, and IFN-mediated antiviral activities. Blocks also the phosphorylation and subsequent activation of IRF3 and IRF7 kinases that are required for interferon-alpha gene expression. Inhibits NF-kappa-B activation and the ubiquitin-like protein ISG15, which is an early antiviral protein. The binding with host ISG15 subsequently blocks host ISGylation. Inhibits ZBP1-dependent necroptosis via interaction with host ZBP1.
Protein Families
Poxviridae E3 protein family
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