EPFL9 Antibody

Code CSB-PA879855XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) EPFL9 Polyclonal antibody
Uniprot No.
Target Names
EPFL9
Alternative Names
EPFL9 antibody; STOMAGEN antibody; At4g12970 antibody; F25G13.60EPIDERMAL PATTERNING FACTOR-like protein 9 antibody; EPF-like protein 9) [Cleaved into: Stomagen] antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana EPFL9 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Positively regulates stomatal density and patterning. Acts by competing with EPF2 (AC Q8LC53) for the same receptors, ERECTA (AC Q42371) and TMM (AC Q9SSD1). Not cleaved by the protease CRSP (AC Q9LNU1).
Gene References into Functions
  1. Analysis of gain and loss of function of STOMAGEN detailed its function as a positive regulator of stomatal formation on both sides of the leaf, not only in terms of stomatal density across the leaf surface but also in terms of their stomatal index. PMID: 26002974
  2. MONOPTEROS directly binds to the STOMAGEN promoter to suppress its expression in mesophyll and inhibit stomatal development. PMID: 25002510
  3. control of stomatal density by the STOMAGEN gene PMID: 23432385
  4. This study provides evidence of a mesophyll-derived positive regulator of stomatal development, called stomagen. [stomagen] PMID: 20010603
  5. We propose that stomagen is a mesophyll-to-epidermis signaling molecule that positively regulates stomatal density. [STOMAGEN] PMID: 20007289
  6. EPFL9 plays a role in controlling stomatal development.[EPFL9] PMID: 20149115

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Subcellular Location
Secreted, extracellular space, apoplast. Secreted.
Protein Families
Plant cysteine rich small secretory peptide family, Epidermal patterning factor subfamily
Tissue Specificity
Expressed in immature organs, including leaves, stems and flower buds, but not in roots, shoot apical meristem and petals. Detected in the mesophyll tissues but not in the epidermal tissues where stomata develop.
Database Links

KEGG: ath:AT4G12970

STRING: 3702.AT4G12970.1

UniGene: At.43880

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