EPTA Antibody

Datasheet

Code	CSB-PA335584XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: EPTA antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34.1 kDa Observed band size: 34 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) eptA Polyclonal antibody

Uniprot No.

P30845

Target Names

eptA

Alternative Names

eptA; pmrC; yjdB; b4114; JW5730; Phosphoethanolamine transferase EptA; Polymyxin resistance protein PmrC

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) EPTA protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the EPTA polyclonal antibody, recombinant Escherichia coli (strain K12) EPTA protein is introduced into a rabbit. This stimulates the rabbit to produce antibodies that specifically target this antigen. Antibody production is further enhanced by a booster injection. The resulting EPTA polyclonal antibodies in the rabbit's serum are then collected and purified using protein A/G. Rigorous testing in ELISA and WB assays has confirmed that the EPTA antibody specifically recognizes Escherichia coli (strain K12) EPTA protein.

The Escherichia coli (strain K12) EPTA protein is an enzyme involved in the modification of lipopolysaccharides (LPS) in the outer membrane of the bacterium. EPTA transfers phosphoethanolamine (PEA) groups to the lipid A portion of LPS, leading to the modification of its structure, thus providing resistance against certain antimicrobial peptides and polymyxins.

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Target Background

Function

Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin.

Gene References into Functions

Data suggest that the plasmid-encoded phosphoethanolamine transferase MCR-1-harboring plasmid is playing an important role in spreading this mechanism of resistance to polymyxins. PMID: 28678874
YjdB, which co-localizes with the ZipA protein, is involved in cell division PMID: 16079137

Subcellular Location

Cell inner membrane; Multi-pass membrane protein.

Protein Families

Phosphoethanolamine transferase family, EptA subfamily

Database Links

KEGG: ecj:JW5730

STRING: 316385.ECDH10B_4306

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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EPTA Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background