HTR4 Antibody

Code CSB-PA010109XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) HTR4 Polyclonal antibody
Uniprot No.
Target Names
Alternative Names
HTR4 antibody; At4g40030 antibody; T5J17.200; antibody; HTR5 antibody; At4g40040 antibody; T5J17.210; antibody; HTR8 antibody; At5g10980 antibody; T30N20_250 antibody; T5K6.6Histone H3.3 antibody; Histone H3.2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana HTR4 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Gene References into Functions
  1. Gene body methylation is likely shaped by H3.3 dynamics in conjunction with transcriptional activity. PMID: 28521766
  2. Clustered pathways are characterized by distinct chromatin signatures of H3K27me3 and H2A.Z, associated with cluster repression and activation, respectively, and represent discrete windows of co-regulation in the genome. PMID: 26895889
Subcellular Location
Nucleus. Chromosome. Nucleus, nucleolus.
Protein Families
Histone H3 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT4G40030

STRING: 3702.AT5G10980.1

UniGene: At.10968

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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