KLHDC3 Antibody

Datasheet

Code	CSB-PA858414ZA01HU
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: KLHDC3 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52 kDa Observed band size: 52 kDa
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Product Details

Uniprot No.

Q9BQ90

Target Names

KLHDC3

Alternative Names

Kelch domain-containing protein 3 (Protein Peas) (Testis intracellular mediator protein), KLHDC3, PEAS

Species Reactivity

Homo sapiens

Immunogen

Recombinant Homo sapiens KLHDC3 protein (1-382aa)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the KLHDC3 polyclonal antibody begins with the selection of a recombinant human KLHDC3 protein segment (amino acids 1-382) as the immunogen. This protein is employed to immunize a rabbit, resulting in an antibody response. The serum is then collected from the rabbit to extract polyclonal antibodies. These antibodies are subsequently purified via affinity chromatography, isolating them based on their specific reactivity to KLHDC3 protein. A thorough validation process confirms the antibody's specificity for human KLHDC3 protein in ELISA and WB assays.

KLHDC3 acts as an adaptor protein within the CRL2 complex, forming interactions with the C-terminal degron of p14^ARF. This interaction leads to the proteasomal degradation of p14^ARF in various cancer cell lines. Consequently, the CRL2-KLHDC3 E3 complex alleviates p14^ARF-mediated inhibition of SLC7A11 transcription, thereby suppressing ferroptosis and promoting the pro-tumorigenic functions of KLHDC3.

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Target Background

Function

Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. The C-degron recognized by the DesCEND pathway is usually a motif of less than ten residues and can be present in full-length proteins, truncated proteins or proteolytically cleaved forms. The CRL2(KLHDC3) complex specifically recognizes proteins with a glycine (Gly) at the C-terminus, leading to their ubiquitination and degradation: recognizes the C-terminal -Arg-(Xaa)n-Arg-Gly, -Arg-(Xaa)n-Lys-Gly, and -Arg-(Xaa)n-Gln-Gly degrons. The CRL2(KLHDC3) complex mediates ubiquitination and degradation of truncated SELENOV and SEPHS2 selenoproteins produced by failed UGA/Sec decoding, which end with a glycine. May be involved in meiotic recombination process.

Gene References into Functions

cloned human and mouse Peas cDNAs (hPEAS/mPeas) and analyzed their tissue and stage-specific expressions; may be involved in meiotic recombination process PMID: 12606021

Subcellular Location

Cytoplasm.

Database Links

HGNC: 20704

OMIM: 611248

KEGG: hsa:116138

STRING: 9606.ENSP00000313995

UniGene: Hs.412468

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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KLHDC3 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background