prkci Antibody

Code CSB-PA845883XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) prkci Polyclonal antibody
Uniprot No.
Target Names
prkci
Alternative Names
prkci antibody; halProtein kinase C iota type antibody; EC 2.7.11.13 antibody; Atypical protein kinase C-lambda/iota antibody; aPKC-lambda/iota antibody; Heart and soul protein antibody; nPKC-iota antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) prkci protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is required for the formation and maintenance of the zonula adherens during early epithelial development and plays a critical role in organ morphogenesis and in regulating the orientation of cell division.
Gene References into Functions
  1. aPKC regulates apical localization of Lgl1 and Lgl2 to restrict elongation of microridges in developing zebrafish epidermis. PMID: 27249668
  2. Zebrafish pronephros tubulogenesis and epithelial identity maintenance are reliant on the polarity proteins Prkc iota and zeta. PMID: 25446529
  3. a loss of zBves affects the proteins involved in the pathway of the PAR junctional complex, especially aPKC, and both aPKC and Bves are indispensable to claudin expression. PMID: 23019331
  4. These results suggest that the aPKC cell autonomously controls the Golgi localization and thereby regulates the specification of the primary dendrite of Purkinje cells. PMID: 21159968
  5. Heart and soul/PRKCi and nagie oko/Mpp5 regulate myocardial coherence and remodeling during cardiac morphogenesis. PMID: 16319113
  6. PrkCi function and planar divisions are necessary for asymmetric, self-renewing division of spinal cord precursors. PMID: 19449304

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Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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