map Antibody

Datasheet

Code	CSB-PA360042XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: map Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) map Polyclonal antibody

Uniprot No.

P0AE18

Target Names

map

Alternative Names

Methionine aminopeptidase (MAP) (MetAP) (EC 3.4.11.18) (Peptidase M) map b0168 JW0163

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) map protein (2-264aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Requires deformylation of the N(alpha)-formylated initiator methionine before it can be hydrolyzed.

Gene References into Functions

These data suggest that map expression contributes to the maintenance of enteropathogenic Escherichia coli colonization. PMID: 25312947
Methionine aminopeptidase associates with ribosomes via a charged loop crucial for nascent-chain processing & cell viability. It competes with peptide deformylase for binding sites at the ribosomal tunnel exit PMID: 23770820
Data show that Europium ion (Eu(3+)) binds to methionine aminopeptidase and binding results in the activation of the enzyme. PMID: 22112844
obtained a crystal structure for a complex of Escherichia coli methionine aminopeptidase with norleucine phosphonate, a transition-state analog, and only a single Mn(II) ion bound at the active site PMID: 16769889

Protein Families

Peptidase M24A family, Methionine aminopeptidase type 1 subfamily

Database Links

KEGG: ecj:JW0163

STRING: 316385.ECDH10B_0147

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map Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background