N Antibody

Datasheet

Code	CSB-PA322753ZA01HIT
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: N antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 49.4 kDa Observed band size: 60 kDa
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Target Background

Product Details

Uniprot No.

P15130

Target Names

Alternative Names

Nucleoprotein (Nucleocapsid protein) (NC) (Protein N), N

Species Reactivity

Human coronavirus 229E

Immunogen

Recombinant Human coronavirus 229E Nucleoprotein(N) (1-389aa)

Immunogen Species

Human coronavirus 229E

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

In the generation of the N antibody, a rabbit is immunized with recombinant human coronavirus 229E nucleoprotein (N) (1-389aa), resulting in the production of IgG antibodies by the rabbit's B lymphocytes. The polyclonal N antibody is then subjected to a meticulous purification process using the protein G. This antibody has been tested for its effective binding with the human coronavirus 229E nucleoprotein in ELISA and WB tests.

The human coronavirus 229E nucleoprotein mainly binds and encapsulates the viral RNA, playing a crucial role in the replication and transcription of the viral genome. The nucleoprotein helps protect the viral RNA from degradation and serves as a structural component of the viral ribonucleoprotein complex. Additionally, it interacts with other viral and host factors to facilitate the assembly of new viral particles.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication.

Gene References into Functions

N protein mutants with truncated C-terminal domains were generated and investigated by biophysical and biochemical analyses to clarify the role of the C-terminal tail of the N protein in oligomerization PMID: 23178926
Nucleocapsid protein expression facilitates coronavirus replication PMID: 17037502

Subcellular Location

Virion. Host endoplasmic reticulum-Golgi intermediate compartment. Host Golgi apparatus.

Protein Families

Alphacoronavirus nucleocapsid protein family

Database Links

KEGG: vg:918763

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