ogt Antibody

^{Rare Species}

Datasheet

Code	CSB-PA013788XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: ogt Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 25 kDa Observed band size: 25 kDa
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Target Background

Product Details

Uniprot No.

P0AFH0

Target Names

ogt

Alternative Names

Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase) ogt b1335 JW1329

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) ogt protein (1-171aa)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The development of polyclonal antibodies against ogt requires repetitive immunizations of a rabbit with recombinant Escherichia coli (strain K12) ogt protein (1-171aa). Upon achieving a satisfactory antibody titer, the rabbit undergoes bleeding, and antibodies are purified from the serum via affinity chromatography. The resulting ogt antibody is then evaluated for functionality in ELISA and WB applications, demonstrating its capability to recognize Escherichia coli (strain K12) ogt protein.

The ogt protein in Escherichia coli (strain K12) plays a crucial role in DNA repair. Its main function involves the removal of alkyl groups, such as methyl or ethyl groups, from the O6 position of guanine in DNA. This repair activity is targeted at addressing DNA damage caused by exposure to alkylating agents, both endogenous and exogenous, thereby preserving the integrity of the DNA molecule. By detoxifying alkylating agents and maintaining genomic stability, ogt contributes to the cell's ability to cope with environmental stresses and challenges.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.

Subcellular Location

Cytoplasm.

Protein Families

MGMT family

Database Links

KEGG: ecj:JW1329

STRING: 316385.ECDH10B_1455

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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ogt Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background