Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TENT4B Polyclonal antibody

Uniprot No.

Q8NDF8

Target Names

TENT4B

Alternative Names

TENT4B antibody; GLD4 antibody; PAPD5 antibody; TRF4-2 antibody; TUT3 antibody; Terminal nucleotidyltransferase 4B antibody; Non-canonical poly(A) RNA polymerase PAPD5 antibody; EC 2.7.7.19 antibody; PAP-associated domain-containing protein 5 antibody; Terminal guanylyltransferase antibody; EC 2.7.7.- antibody; Terminal uridylyltransferase 3 antibody; TUTase 3 antibody; Topoisomerase-related function protein 4-2 antibody; TRF4-2 antibody

Raised in

Rabbit

Species Reactivity

Homo sapiens

Immunogen

Recombinant Homo sapiens TENT4B protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Target Background

Function

Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation.

Gene References into Functions

TENT4A and TENT4B produce a mixed tail that shields mRNA from rapid deadenylation. PMID: 30026317
A novel post-transcriptional regulatory network involving carbohydrate metabolism and glucose homeostasis mediated by GLD4. PMID: 28383716
We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly PMID: 27434818
PAPD5 and PARN mediate degradation of oncogenic miRNA miR-21. PMID: 25049417
PAPD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. PMID: 21788334

Hide All

Subcellular Location

Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165).

Protein Families

DNA polymerase type-B-like family

Database Links

HGNC: 30758

OMIM: 605540

KEGG: hsa:64282

STRING: 9606.ENSP00000396995

UniGene: Hs.514342

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TENT4B Antibody

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