Recombinant Human Terminal nucleotidyltransferase 4B (TENT4B)

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Code CSB-EP843329HU
Size US$306
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP843329HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TENT4B.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP843329HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) TENT4B.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
PAPD5
Uniprot No.
Research Area
Epigenetics and Nuclear Signaling
Alternative Names
TENT4B; GLD4; PAPD5; TRF4-2; TUT3; Terminal nucleotidyltransferase 4B; Non-canonical poly(A) RNA polymerase PAPD5; EC 2.7.7.19; PAP-associated domain-containing protein 5; Terminal guanylyltransferase; EC 2.7.7.-; Terminal uridylyltransferase 3; TUTase 3; Topoisomerase-related function protein 4-2; TRF4-2
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-572aa
Target Protein Sequence
MYRSGERLLGSHALPAEQRDFLPLETTNNNNNHHQPGAWARRAGSSASSPPSASSSPHPSAAVPAADPADSASGSSNKRKRDNKASGGRAAGGGRADGGGVVYSGTPWKRRNYNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFGSFKTGLYLPTSDIDLVVFGKWENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKLTDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIGSYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAKDEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNETESILGRIIRVTDEVATYRDWISKQWGLKNRPEPSCNGPVSSSSATQSSSSDVDSDATPCKTPKQLLCRPSTGNRVGSQDVSLESSQAVGKMQSTQTTNTSNSTNKSQHGSARLFRSSSKGFQGTTQTSHGSLMTNKQHQGKSNNQYYHGKKRKHKRDAPLSDLCR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
67.3 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Regarding your protein CSB-EP843329HU, could you please advise on the lead time, the sequencing, the pricing, and the expected tag information?
Also, do you have any customer-reported information as to whether or not this protein has retained any activity?
Thanks!

A:
Thanks for your inquiry.
Recombinant Human PAP-associated domain-containing protein 5(PAPD5)
CSB-EP843329HUa2 >> E.coli N-terminal 6xHis-SUMO-tagged
CSB-EP843329HU >> E.coli N-terminal 6xHis-tagged
Expression Region: 1-572aa; Full length.
Sequence:

MYRSGERLLGSHALPAEQRDFLPLETTNNNNNHHQPGAWARRAGSSASSPPSASSSPHPSAAVPAADPADSASGSSNKRKRDNKASGGRAAGGGRADGGGVVYSGTPWKRRNYNQGVVGLHEEISDFYEYMSPRPEEEKMRMEVVNRIESVIKELWPSADVQIFGSFKTGLYLPTSDIDLVVFGKWENLPLWTLEEALRKHKVADEDSVKVLDKATVPIIKLTDSFTEVKVDISFNVQNGVRAADLIKDFTKKYPVLPYLVLVLKQFLLQRDLNEVFTGGIGSYSLFLMAVSFLQLHPREDACIPNTNYGVLLIEFFELYGRHFNYLKTGIRIKDGGSYVAKDEVQKNMLDGYRPSMLYIEDPLQPGNDVGRSSYGAMQVKQAFDYAYVVLSHAVSPIAKYYPNNETESILGRIIRVTDEVATYRDWISKQWGLKNRPEPSCNGPVSSSSATQSSSSDVDSDATPCKTPKQLLCRPSTGNRVGSQDVSLESSQAVGKMQSTQTTNTSNSTNKSQHGSARLFRSSSKGFQGTTQTSHGSLMTNKQHQGKSNNQYYHGKKRKHKRDAPLSDLCR


Regarding the activity. sorry, we haven't tested its activity and also haven't received the feedback about the activity from other customers.
In theory, what we provide is the full length protein, we adpot affinity chromatography for purification and it's purified under very mild condition,
it's very likely to be active, we recommend you to purchase a small size to have a try. The EP protein has been ordered many times by other customers.
Here is a literature for your reference: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3162338/
It is mentioned in this literature that recombinant PAPD5 expressed in mammalian cells and bacteria is active in the absence of protein cofactors.
We analyzed the in vitro properties of recombinant PAPD5 expressed in mammalian cells as well as in bacteria.
Our results show that PAPD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. Interestingly, PAPD5 is active without a protein cofactor.

Target Background

Function
Terminal nucleotidyltransferase that catalyzes preferentially the transfert of ATP and GTP on RNA 3' poly(A) tail creating a heterogeneous 3' poly(A) tail leading to mRNAs stabilization by protecting mRNAs from active deadenylation. Also functions as a catalytic subunit of a TRAMP-like complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo(A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Doesn't need a cofactor for polyadenylation activity (in vitro). Required for cytoplasmic polyadenylation of mRNAs involved in carbohydrate metabolism, including the glucose transporter SLC2A1/GLUT1. Plays a role in replication-dependent histone mRNA degradation, probably through terminal uridylation of mature histone mRNAs. May play a role in sister chromatid cohesion. Mediates 3' adenylation of the microRNA MIR21 followed by its 3'-to-5' trimming by the exoribonuclease PARN leading to degradation. Mediates 3' adenylation of H/ACA box snoRNAs (small nucleolar RNAs) followed by its 3'-to-5' trimming by the exoribonuclease PARN which enhances snoRNA stability and maturation.
Gene References into Functions
  1. TENT4A and TENT4B produce a mixed tail that shields mRNA from rapid deadenylation. PMID: 30026317
  2. A novel post-transcriptional regulatory network involving carbohydrate metabolism and glucose homeostasis mediated by GLD4. PMID: 28383716
  3. We analyzed the interactions of the human TRAMP-like proteins, PAPD5, ZCCHC7, and MTR4, with the nuclear exosome. PAPD5 and ZCCHC7 exhibited mutual interactions in presence of the exosome catalytic subunit RRP6, whereas MTR4 was dispensable for their assembly PMID: 27434818
  4. PAPD5 and PARN mediate degradation of oncogenic miRNA miR-21. PMID: 25049417
  5. PAPD5 catalyzes the polyadenylation of different types of RNA substrates in vitro. PMID: 21788334

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Subcellular Location
Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Predominantly expressed in the cytoplasm (PubMed:18172165).
Protein Families
DNA polymerase type-B-like family
Database Links

HGNC: 30758

OMIM: 605540

KEGG: hsa:64282

STRING: 9606.ENSP00000396995

UniGene: Hs.514342

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