DAD1 Antibody

Code CSB-PA850075XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) DAD1 Polyclonal antibody
Uniprot No.
Target Names
DAD1
Alternative Names
DAD1 antibody; At2g44810 antibody; T13E15.18 antibody; Phospholipase A(1) DAD1 antibody; chloroplastic antibody; EC 3.1.1.32 antibody; Phospholipase A1-Ibeta1 antibody; Protein DEFECTIVE IN ANTHER DEHISCENCE 1 antibody; AtDAD1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana DAD1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Sn-1-specific phospholipase that releases free fatty acids from phospholipids. Low activity on galactolipids and triacylglycerols. Catalyzes the initial step of jasmonic acid biosynthesis. Not essential for jasmonate biosynthesis after wounding or upon pathogen infection.
Gene References into Functions
  1. The expression of DAD1, DGL, and other DAD1-LIKE LIPASE (DALL) genes is induced upon wounding. DAD1 and DGL required COI1 for their expression, suggesting that a molecule other than jasmonic acid (JA) might act as a regulator of COI1. PMID: 24430866
  2. participates in jasmonate formation only in the late wound response PMID: 20348210
Subcellular Location
Plastid, chloroplast.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Expressed in flower buds, but not in leaves or roots. Restricted to the stamen filaments immediately before flower opening.
Database Links

KEGG: ath:AT2G44810

STRING: 3702.AT2G44810.1

UniGene: At.37029

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