Recombinant Arabidopsis thaliana Phospholipase A (1) DAD1, chloroplastic (DAD1)

Code CSB-YP850075DOA
MSDS
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Source Yeast
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Code CSB-EP850075DOA
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Source E.coli
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Code CSB-EP850075DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP850075DOA
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Source Baculovirus
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Code CSB-MP850075DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DAD1
Uniprot No.
Alternative Names
DAD1; At2g44810; T13E15.18; Phospholipase A(1) DAD1; chloroplastic; EC 3.1.1.32; Phospholipase A1-Ibeta1; Protein DEFECTIVE IN ANTHER DEHISCENCE 1; AtDAD1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
47-447
Target Protein Sequence
VSTS RPPVLPKPET WESLLLNHDQ IPGEFSPTGS SIPVKLGRRW MEYQGLQNWD GLLDPLDDNL RREILRYGQF VESAYQAFDF DPSSPTYGTC RFPRSTLLER SGLPNSGYRL TKNLRATSGI NLPRWIEKAP SWMATQSSWI GYVAVCQDKE EISRLGRRDV VISFRGTATC LEWLENLRAT LTHLPNGPTG ANLNGSNSGP MVESGFLSLY TSGVHSLRDM VREEIARLLQ SYGDEPLSVT ITGHSLGAAI ATLAAYDIKT TFKRAPMVTV ISFGGPRVGN RCFRKLLEKQ GTKVLRIVNS DDVITKVPGV VLENREQDNV KMTASIMPSW IQRRVEETPW VYAEIGKELR LSSRDSPHLS SINVATCHEL KTYLHLVDGF VSSTCPFRET ARRVLHR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Sn-1-specific phospholipase that releases free fatty acids from phospholipids. Low activity on galactolipids and triacylglycerols. Catalyzes the initial step of jasmonic acid biosynthesis. Not essential for jasmonate biosynthesis after wounding or upon pathogen infection.
Gene References into Functions
  1. The expression of DAD1, DGL, and other DAD1-LIKE LIPASE (DALL) genes is induced upon wounding. DAD1 and DGL required COI1 for their expression, suggesting that a molecule other than jasmonic acid (JA) might act as a regulator of COI1. PMID: 24430866
  2. participates in jasmonate formation only in the late wound response PMID: 20348210
Subcellular Location
Plastid, chloroplast.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Expressed in flower buds, but not in leaves or roots. Restricted to the stamen filaments immediately before flower opening.
Database Links

KEGG: ath:AT2G44810

STRING: 3702.AT2G44810.1

UniGene: At.37029

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