Phospholipase A2 Antibody

Code CSB-PA018091ZA01DNK
Size US$299
Image
  • Western Blot
    Positive WB detected in: recombinant protein
    All lanes: Phospholipase A2 Antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 20 kDa
    Observed band size: 20 kDa
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Product Details

Full Product Name
Rabbit anti-Apis mellifera (Honeybee) Phospholipase A2 Polyclonal antibody
Uniprot No.
Target Names
Phospholipase A2
Alternative Names
Phospholipase A2 (bvPLA2) (EC 3.1.1.4) (Allergen Api m I) (Phosphatidylcholine 2-acylhydrolase) (allergen Api m 1)
Raised in
Rabbit
Species Reactivity
Apis mellifera
Immunogen
Recombinant Apis mellifera Phospholipase A2 protein (34-167)
Immunogen Species
Apis mellifera (Honeybee)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.
Gene References into Functions
  1. PLA(2)s were located on the membrane surface rather than penetrating the membrane bilayer and that the deeply inserted mode is not a favorable condition for the hydrolysis of phospholipids in zwitterionic phospholipid membranes. PMID: 21557547
  2. contributions of the individual disulfide bonds to conformational stability PMID: 20884319
  3. successive introduction of sequence modifications outside the human and murine T cell epitope regions led to a progressive loss of specific IgE and IgG reactivity PMID: 15459335
  4. Studies with bee venom (bv) mutant PLA2 show that electrostatic interactions between lysine and arginine residues and anionic phosphatidylserine contribute minimally to overall interfacial binding energetics of bvPLA2. PMID: 15491136
Subcellular Location
Secreted.
Protein Families
Phospholipase A2 family, Group III subfamily
Tissue Specificity
Expressed by the venom gland.
Database Links

KEGG: ame:406141

STRING: 7460.GB13351-PA

UniGene: Ame.2

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