RCSF Antibody

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Datasheet

Code	CSB-PA303145XA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: RCSF antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39.3 kDa Observed band size: 37 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) rcsF Polyclonal antibody

Uniprot No.

P69411

Target Names

rcsF

Alternative Names

rcsF antibody; b0196 antibody; JW0192Outer membrane lipoprotein RcsF antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) RCSF protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The RCSF antibody is created by immunizing a rabbit with the recombinant Escherichia coli (strain K12) RCSF protein. It is then purified using protein A/G affinity chromatography. This antibody is recommended for detecting the Escherichia coli (strain K12) RCSF protein in ELISA and WB assays.

The Escherichia coli (strain K12) RCSF protein is crucial for maintaining the integrity of the cell envelope and coordinating the response to environmental stresses in Escherichia coli (strain K12). It is involved in the activation of the RcsC/RcsD/RcsB phosphorelay system that plays a role in controlling the production of capsular polysaccharides and regulates the expression of various genes involved in cell envelope synthesis, stress response, and virulence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential component of the Rcs signaling system, which controls transcription of numerous genes. Plays a role in signal transduction from the cell surface to the histidine kinase RcsC. May detect outer membrane defects. The system controls expression of genes involved in colanic acid capsule synthesis, biofilm formation and cell division.

Gene References into Functions

RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system. PMID: 28691662
Here, the authors show that the RcsF/outer membrane protein complex is functional and that lipopolysaccharide defects are sensed directly by the surface-exposed domain of RcsF. PMID: 27282389
The proline-rich region of RcsF regulated the function of RcsF in activating the Rcs system. PMID: 23813676
Mislocalization of RcsF activates the Rcs system. It seems that RcsF must be localized in the outer membrane to respond effectively to stimuli from outside the cell. PMID: 22322964

Subcellular Location

Cell outer membrane; Lipid-anchor; Periplasmic side.

Protein Families

RcsF family

Database Links

KEGG: ecj:JW0192

STRING: 316385.ECDH10B_0177

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RCSF Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background