rif1 Antibody

Code CSB-PA839423XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rif1 Polyclonal antibody
Uniprot No.
Target Names
rif1
Alternative Names
rif1 antibody; SPAC6F6.17 antibody; SPAPJ736.01 antibody; Telomere length regulator protein rif1 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) rif1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Negatively regulates telomere length. Appears to play no role in transcriptional silencing of telomeric loci.
Gene References into Functions
  1. Genetic screens for identifying cis- and trans-acting factors creating heterochromatin-euchromatin boundaries showed unexpected properties for Rif1 and Taz1. Most cis-acting elements identified came from genomic loci bound by Taz1 and associated with origins of replication repressed by Taz1 and Rif1. Boundary formation by these elements required Taz1 and Rif1 and coincided with altered replication timing. PMID: 28096402
  2. Rif1 is a critical determinant of the origin activation program on the fission yeast chromosomes [Rif1] [strain not found in database] PMID: 22279046
  3. Rif1 localizes to the anaphase mid-region and regulates the resolution of persisting DNA structures. PMID: 27320927
  4. These results suggest that Rif1 recognizes and binds G quadruplex-like structures at selected intergenic regions, thus generating local chromatin structures that may exert long-range suppressive effects on origin firing. PMID: 26436827
  5. Rap1 and Rif1 are each required for a subset of the functions of Taz1 PMID: 16096639

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Subcellular Location
Nucleus. Chromosome, telomere. Note=Localized to telomeres. This interaction may be increased by perturbation of telomere structure, for instance by loss of the rap1 protein.
Protein Families
RIF1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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