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Recombinant Schizosaccharomyces pombe Telomere length regulator protein rif1 (rif1), partial

Recombinant Schizosaccharomyces pombe Telomere length regulator protein rif1 (rif1), partial

Code	CSB-YP839423SXV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP839423SXV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP839423SXV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP839423SXV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP839423SXV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

rif1

Uniprot No.

Q96UP3

Alternative Names

rif1; SPAC6F6.17; SPAPJ736.01; Telomere length regulator protein rif1

Species

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Negatively regulates telomere length. Appears to play no role in transcriptional silencing of telomeric loci.

Gene References into Functions

Genetic screens for identifying cis- and trans-acting factors creating heterochromatin-euchromatin boundaries showed unexpected properties for Rif1 and Taz1. Most cis-acting elements identified came from genomic loci bound by Taz1 and associated with origins of replication repressed by Taz1 and Rif1. Boundary formation by these elements required Taz1 and Rif1 and coincided with altered replication timing. PMID: 28096402
Rif1 is a critical determinant of the origin activation program on the fission yeast chromosomes [Rif1] [strain not found in database] PMID: 22279046
Rif1 localizes to the anaphase mid-region and regulates the resolution of persisting DNA structures. PMID: 27320927
These results suggest that Rif1 recognizes and binds G quadruplex-like structures at selected intergenic regions, thus generating local chromatin structures that may exert long-range suppressive effects on origin firing. PMID: 26436827
Rap1 and Rif1 are each required for a subset of the functions of Taz1 PMID: 16096639

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Subcellular Location

Nucleus. Chromosome, telomere. Note=Localized to telomeres. This interaction may be increased by perturbation of telomere structure, for instance by loss of the rap1 protein.

Protein Families

RIF1 family

Database Links

KEGG: spo:SPAC6F6.17

STRING: 4896.SPAC6F6.17.1

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