SKP1 Antibody

Datasheet

Code	CSB-PA347310XA01SVG
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Target Background

Product Details

Full Product Name

Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SKP1 Polyclonal antibody

Uniprot No.

P52286

Target Names

SKP1

Alternative Names

SKP1 antibody; CBF3D antibody; YDR328C antibody; D9798.14 antibody; Suppressor of kinetochore protein 1 antibody; Centromere DNA-binding protein complex CBF3 subunit D antibody; E3 ubiquitin ligase complex SCF subunit SKP1 antibody

Raised in

Rabbit

Species Reactivity

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Immunogen

Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) SKP1 protein

Immunogen Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK-like proteins into the kinetochore thus providing cell cycle-regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52.

Gene References into Functions

findings revealed that Rok1 protein synthesis is repressed by Psk2 and Skp1; this repression appeared to be mediated through the ROK1 uORF1 PMID: 26310304
Data show that the amino terminus of Sgt1p interacts with CBF3 subunits Skp1p and Ctf13p. PMID: 15456868
Sgt1p acts as a "client adaptor" recruiting specific clients to Hsp82p co-chaperone complexes PMID: 16945921
Skp1p does not appear to be involved in V-ATPase assembly but instead in the cycling of the complex off membranes PMID: 17041187
findings suggest a novel function of Skp1 in mitotic exit and cytokinesis, independent of its function as a part of the SCF complex; interaction of Skp1 and Bfa1 may contribute to the function of Skp1 in the mitotic exit PMID: 17205042
Saf1p is being a bona fide Skp1-Cullin-F-box subunit PMID: 17517885

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Subcellular Location

Cytoplasm. Nucleus. Chromosome, centromere, kinetochore.

Protein Families

SKP1 family

Database Links

KEGG: sce:YDR328C

STRING: 4932.YDR328C

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

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Related pathways

Wnt signaling pathway Cell cycle Protein processing in endoplasmic reticulum Ubiquitin mediated proteolysis TGF-beta signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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SKP1 Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background