Trim63 Antibody

Code CSB-PA856332ZA01RA
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Product Details

Full Product Name
Rabbit anti-Rattus norvegicus (Rat) Trim63 Polyclonal antibody
Uniprot No.
Target Names
Trim63
Alternative Names
E3 ubiquitin-protein ligase TRIM63 (EC 2.3.2.27) (Muscle-specific RING finger protein 1) (MuRF-1) (MuRF1) (RING-type E3 ubiquitin transferase TRIM63) (Tripartite motif-containing protein 63), Trim63, Murf1
Raised in
Rabbit
Species Reactivity
Rattus norvegicus
Immunogen
Recombinant Rattus norvegicus Trim63 protein
Immunogen Species
Rattus norvegicus (Rat)
Conjugate
Non-conjugate
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (12-14 weeks)

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Target Background

Function
E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.
Gene References into Functions
  1. Exercise down-regulated the mRNA levels of murf1 and atrogin-1, decreased reactive oxygen species level, increased antioxidant capacity, regulated the expression of insulin-like growth factor 1 (IGF1), mechano growth factor (MGF), Neuregulin1 (NRG1) and Myostatin (MSTN), and activated Akt and Erk1/2 signalings in soleus muscle. PMID: 30312703
  2. UBE2D2 is not the cognate ubiquitinating enzyme for MuRF1 PMID: 27378730
  3. Data suggest that FOXO3a plays a role in muscle atrophy through regulation of expression of MuRF1 and atrogin-1 in skeletal muscle in Cushing's syndrome. (FOXO3a = forkhead box protein O3a; MuRF1 = muscle-specific RING finger protein 1) PMID: 28246104
  4. Independent of the metabolic condition, after muscle contraction, both TRIM63 and FBXO32 proteins correlated significantly with miR-1-3p, miR-29a/b-3p, and miR-133a/b-3p. PMID: 28000044
  5. The muscles also showed significantly contrasting transcriptional regulation of the microRNAs miR-1 and miR-206. MuRF1 and Atrogin-1 showed the highest levels of expression in the denervated gastrocnemius muscle PMID: 26691660
  6. MuRF1 and MuRF2 translocate to the nuclei in the soleus muscle during gravitational unloading. PMID: 25868327
  7. Data from model of hypertensive heart disease suggest expression of E3 ubiquitin ligases (muscle RING-finger protein-1; atrogin-1) are upregulated in cardiomyocytes as left ventricle hypertrophy develops and down-regulated if hypertrophy prevented. PMID: 24084216
  8. Suppression of atrogin-1 resulted in increased expression of MuRF1. PMID: 23866982
  9. miR-19a/b family regulates phenotypes of cardiomyocytes via suppression of multiple direct target genes such as murf-1. PMID: 24117217
  10. Branched-chain amino acids attenuated atrogin-1 and MuRF1 protein levels induced by hindlimb suspension. PMID: 23084640
  11. Ligustrazine may postpone denervated skeletal muscle atrophy by reducing mRNA and protein expressions of FoXO3a, MAFbx, and MuRF1. PMID: 22702057
  12. Diabetes modulated the expression of MuRF1 leading to muscle wasting, and the mechanism might be involved with oxidative stress. PMID: 21826997
  13. recombinant GST-MuRF1 physically interacted and polyubiquitinylated actin in vitro and that MuRF1 is a critical component for actin breakdown, since MuRF1 siRNA stabilized flag-actin PMID: 21764995
  14. AMPK regulates the transcription of Atrogin-1 and MuRF1 and enhances UPS-mediated protein degradation in heart. PMID: 21921267
  15. Exercise training diminished the skeletal muscle wasting in diabetic rats by decreasing oxidative stress and inhibiting MuRF1 expression at both the mRNA and protein levels. PMID: 21620866
  16. Heart failure induces fiber type IIB specific atrophy, up-regulating atrogin-1 and MuRF1 mRNA expression in extensor digitorum longus muscle of monocrotaline treated rats. PMID: 20349269
  17. Dietary fish oil prior to and during immobilization may alleviate the immobilization-induced soleus muscle atrophy, at least in part, via the Akt pathway through E3 ubiquitin ligases and p70s6k. PMID: 20555375
  18. Persistent over-expression of MAFbx and MuRF1 mRNA after muscle transfer has a close relationship with muscle atrophy and muscle dysfunction. PMID: 19803207
  19. atrogin-1 and MuRF1 are upregulated in experimental hyperthyroidism in rats PMID: 19777444
  20. MafBx and Murf mRNA are up-regulated in response to LPS and might play a role in the muscle proteolysis observed PMID: 12782319
  21. IGF-1/Akt and TNFalpha represent potential mediators implicated in the regulation of MuRF1 and atrogin-1 genes during aging PMID: 16949134
  22. Murf1 in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading is described. PMID: 17622304
  23. The antioxidant Trolox attenuates mechanical ventilation-induced diaphragmatic atrophy independent of alterations in Akt regulation of FoxO transcription factors and expression of MAFbx or MuRF-1. PMID: 17916612
  24. the regulation by glucocorticoids of MuRF1 and the role of the GR may be different in L6 (a rat cell line) and C2C12 (a mouse cell line) myotubes PMID: 18615595
  25. the transcriptional activities of Foxo3a and NF-kappaB, which are implicated in the regulation of atrogin-1 and MuRF1, were abolished by Hsp70 PMID: 18644837
  26. Ghrelin inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx. PMID: 19211729

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Subcellular Location
Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line.
Tissue Specificity
Muscle specific. Selectively expressed in heart and skeletal muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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