Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Trim63

Uniprot No.

Q91Z63

Research Area

Cardiovascular

Alternative Names

Muscle-specific RING finger protein 1 RING-type E3 ubiquitin transferase TRIM63 Tripartite motif-containing protein 63

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

1-351aa

Target Protein Sequence

MDYKSGLIPDGNAMENLEKQLICPICLEMFTKPVVILPCQHNLCRKCANDIFQAANPYWTNRGGSVSMSGGRFRCPSCRHEVIMDRHGVYGLQRNLLVENIIDIYKQECSSRPLQKGSHPMCKEHEDEKINIYCLTCEVPTCSLCKVFGAHQACEVAPLQSIFQGQKTELSNCISMLVAGNDRVQTIISQLEDSCRVTKENSHQVKEELSHKFDALYAILDEKKSELLQRITQEQEEKLDFIEALILQYREQLEKSTKLVETAIQSLDEPGGATFLLSAKPLIKSIVEASKGCQLGKTEQGFENMDYFTLNLEHIAEALRAIDFGTDEEEEFTEEEEEEDQEEGVSTEGHQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

47.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Rat Trim63 protein is made by following sequential steps: inserting a DNA fragment encoding the Rat Trim63 protein (1-351aa) into a plasmid vector, transferring this vector into e.coli cells, selecting the positive cells, culturing positive cells, inducing the protein expression, and collecting the Trim63 protein. The protein features a N-terminal 10xHis tag and C-terminal Myc tag fusion. The recombinant Trim63 protein is subjected to affinity purification and SDS-PAGE analysis. Its purity is over 90%.

Target Background

Function

E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells.

Gene References into Functions

Exercise down-regulated the mRNA levels of murf1 and atrogin-1, decreased reactive oxygen species level, increased antioxidant capacity, regulated the expression of insulin-like growth factor 1 (IGF1), mechano growth factor (MGF), Neuregulin1 (NRG1) and Myostatin (MSTN), and activated Akt and Erk1/2 signalings in soleus muscle. PMID: 30312703
UBE2D2 is not the cognate ubiquitinating enzyme for MuRF1 PMID: 27378730
Data suggest that FOXO3a plays a role in muscle atrophy through regulation of expression of MuRF1 and atrogin-1 in skeletal muscle in Cushing's syndrome. (FOXO3a = forkhead box protein O3a; MuRF1 = muscle-specific RING finger protein 1) PMID: 28246104
Independent of the metabolic condition, after muscle contraction, both TRIM63 and FBXO32 proteins correlated significantly with miR-1-3p, miR-29a/b-3p, and miR-133a/b-3p. PMID: 28000044
The muscles also showed significantly contrasting transcriptional regulation of the microRNAs miR-1 and miR-206. MuRF1 and Atrogin-1 showed the highest levels of expression in the denervated gastrocnemius muscle PMID: 26691660
MuRF1 and MuRF2 translocate to the nuclei in the soleus muscle during gravitational unloading. PMID: 25868327
Data from model of hypertensive heart disease suggest expression of E3 ubiquitin ligases (muscle RING-finger protein-1; atrogin-1) are upregulated in cardiomyocytes as left ventricle hypertrophy develops and down-regulated if hypertrophy prevented. PMID: 24084216
Suppression of atrogin-1 resulted in increased expression of MuRF1. PMID: 23866982
miR-19a/b family regulates phenotypes of cardiomyocytes via suppression of multiple direct target genes such as murf-1. PMID: 24117217
Branched-chain amino acids attenuated atrogin-1 and MuRF1 protein levels induced by hindlimb suspension. PMID: 23084640
Ligustrazine may postpone denervated skeletal muscle atrophy by reducing mRNA and protein expressions of FoXO3a, MAFbx, and MuRF1. PMID: 22702057
Diabetes modulated the expression of MuRF1 leading to muscle wasting, and the mechanism might be involved with oxidative stress. PMID: 21826997
recombinant GST-MuRF1 physically interacted and polyubiquitinylated actin in vitro and that MuRF1 is a critical component for actin breakdown, since MuRF1 siRNA stabilized flag-actin PMID: 21764995
AMPK regulates the transcription of Atrogin-1 and MuRF1 and enhances UPS-mediated protein degradation in heart. PMID: 21921267
Exercise training diminished the skeletal muscle wasting in diabetic rats by decreasing oxidative stress and inhibiting MuRF1 expression at both the mRNA and protein levels. PMID: 21620866
Heart failure induces fiber type IIB specific atrophy, up-regulating atrogin-1 and MuRF1 mRNA expression in extensor digitorum longus muscle of monocrotaline treated rats. PMID: 20349269
Dietary fish oil prior to and during immobilization may alleviate the immobilization-induced soleus muscle atrophy, at least in part, via the Akt pathway through E3 ubiquitin ligases and p70s6k. PMID: 20555375
Persistent over-expression of MAFbx and MuRF1 mRNA after muscle transfer has a close relationship with muscle atrophy and muscle dysfunction. PMID: 19803207
atrogin-1 and MuRF1 are upregulated in experimental hyperthyroidism in rats PMID: 19777444
MafBx and Murf mRNA are up-regulated in response to LPS and might play a role in the muscle proteolysis observed PMID: 12782319
IGF-1/Akt and TNFalpha represent potential mediators implicated in the regulation of MuRF1 and atrogin-1 genes during aging PMID: 16949134
Murf1 in muscle atrophy caused by blocked neuromuscular transmission and muscle unloading is described. PMID: 17622304
The antioxidant Trolox attenuates mechanical ventilation-induced diaphragmatic atrophy independent of alterations in Akt regulation of FoxO transcription factors and expression of MAFbx or MuRF-1. PMID: 17916612
the regulation by glucocorticoids of MuRF1 and the role of the GR may be different in L6 (a rat cell line) and C2C12 (a mouse cell line) myotubes PMID: 18615595
the transcriptional activities of Foxo3a and NF-kappaB, which are implicated in the regulation of atrogin-1 and MuRF1, were abolished by Hsp70 PMID: 18644837
Ghrelin inhibits skeletal muscle protein breakdown in rats with thermal injury through normalizing elevated expression of E3 ubiquitin ligases MuRF1 and MAFbx. PMID: 19211729

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Subcellular Location

Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line.

Tissue Specificity

Muscle specific. Selectively expressed in heart and skeletal muscle.

Database Links

KEGG: rno:140939

STRING: 10116.ENSRNOP00000058869

UniGene: Rn.40636

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Code	CSB-EP856332RA
Abbreviation	Recombinant Rat Trim63 protein
MSDS	MSDS Datasheet
Size	$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat E3 ubiquitin-protein ligase TRIM63 (Trim63)

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