Bovine Apolipoprotein A-I(APOA1) ELISA kit

Instructions
Code CSB-EL001913BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name apolipoprotein A-I
Alternative Names APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
Abbreviation APOA1
Uniprot No. P15497
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 ng/mL-5000 ng/mL
Sensitivity 19.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1000 Average % 90  
Range % 87-94  
1:2000 Average % 96  
Range % 91-100  
1:4000 Average % 93  
Range % 87-97  
1:8000 Average % 100  
Range % 96-103  
Recovery
The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 82-87  
EDTA plasma (n=4) 89 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
5000 0.140 0.143 0.142    
2500 0.285 0.291 0.288    
1250 0.482 0.503 0.493    
625 0.814 0.821 0.818    
312 1.253 1.268 1.261    
156 1.695 1.709 1.702    
78 2.040 2.134 2.087    
0 2.436 2.458 2.447    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
Gene References into Functions
  1. the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
Subcellular Location Secreted
Protein Families Apolipoprotein A1/A4/E family
Tissue Specificity Major protein of plasma HDL, also found in chylomicrons.
Database Links

KEGG: bta:281631

STRING: 9913.ENSBTAP00000002914

UniGene: Bt.49157

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