Recombinant Bovine Apolipoprotein A-I (APOA1), partial

The expression vector recombined with the recombinant DNA was transfected into the yeast cells for expression. The recombinant DNA resulted from the fusion of the gene coding for the 25-265aa of the Bovine APOA1 protein and the N-terminal 6xHis tag gene. The product was purified and isolated to get the recombinant Bovine APOA1 protein with N-terminal 6xHis tag. The purity of this recombinant Bovine APOA1 protein reaches up to 90%. Under SDS-PAGE condition, this recombinant APOA1 protein showed a band with a molecular weight of about 30 kDa on the gel.

APOA1 is a gene encoding a protein named Apolipoprotein A1 (abbreviated APOA1) and belongs Apolipoprotein A1/A4/E family. APOA1 is the major component of high-density lipoprotein (HDL) and plays a central role in reverse cholesterol transport. Because HDL is able to transport cholesterol and certain fats called phospholipids through the bloodstream from the body's tissues to the liver. A study has showed that apoB and apoA1 were simple clinical indicators, and the apoB/apoA1 ratio was closely related with CHD in the overweight group. The apoB/apoA1 ratio increase indicated an increased risk of CHD and may provide some useful information in the differential diagnosis.