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Bovine Cortisol ELISA Kit

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Citations (40) Protocol
Code CSB-E13064B
Size 96T,5×96T,10×96T
Price Request a Quote
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Abbreviation
COR
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
3.12 ng/ml-200 ng/ml
Sensitivity
0.78 ng/ml
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
 
Linearity
 
Recovery
 
Typical Data
 
ELISA Data Analysis
ELISA Standard Curve & Curve Expert software
Troubleshooting
and FAQs
ELISA kit FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

The Bovine Cortisol ELISA Kit enables quantitative detection of bovine Cortisol in serum, plasma, and tissue homogenates. A detection range of 3.12 ng/ml-200 ng/ml and a sensitivity of 0.78 ng/ml ensures accurate results. The kit uses a competitive detection mechanism, where the Cortisol in the sample competes with a labeled antigen for binding to a limited number of antibody sites. This competition allows for the quantification of the antigen based on the inverse relationship between antigen concentration and signal output. The kit requires 50-100 μL of sample, has an assay time of 1-5 hours, and detection is carried out at 450 nm wavelength. It has been used in scientific experiments documented in more than 39 papers.

Citations

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Customer Reviews and Q&A

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■ Q&A
Q:

Please provide the following information regarding Bovine Cortisol ELISA Kit (CSB-E13064B):
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What's is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
4. If recombinant protein, is it full length or partial?
5. What is the species of the standard– human or something else?
6. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry.
Cortisol is small molecule and it is the same in all species, so there is no uniprot.
Since for different species, the matrix is different, we have developed kits for different species.
CSB-E13064B
The standard is purified cortisol. This is a competitive kit. The plate is coated with specific antigen. The specific antibody is rabbit polyclonal antibody. The immunogen is modified by special modification.
HRP is conjugated with secondary antibody( goat anti-rabbit antibody).
Pls let me know if you have any further questions. Thank you.
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