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Bovine Cortisol ELISA Kit

Instructions
Code CSB-E13064B
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Cortisol
Abbreviation COR
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-99  
1:2 Average % 96  
Range % 92-105  
1:4 Average % 85  
Range % 81-89  
1:8 Average % 91  
Range % 83-98  
Recovery
The recovery of bovine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-95  
EDTA plasma (n=4) 96 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.194 0.184 0.189    
50 0.328 0.320 0.324    
12.5 0.507 0.550 0.529    
3.12 0.900 0.975 0.938    
0.78 1.314 1.266 1.290    
0.195 1.653 1.657 1.655    
0.049 1.915 1.902 1.909    
0 2.306 2.400 2.353    
Troubleshooting
and FAQs		 ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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■ Q&A
Q:

Please provide the following information regarding Bovine Cortisol ELISA Kit (CSB-E13064B):
1. What is the Uniprot/Accession number that this kit is designed for? Is the kit designed for XYZ species or is it designed for a different species such as Human and cross reacts with XYZ species?
2. Is the capture antibody a monoclonal or polyclonal?
3. What's is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
4. If recombinant protein, is it full length or partial?
5. What is the species of the standard– human or something else?
6. What sample type was tested with this kit (serum? plasma? Something else?) and what were the reference values.

A:
Thanks for your inquiry.
Cortisol is small molecule and it is the same in all species, so there is no uniprot.
Since for different species, the matrix is different, we have developed kits for different species.
CSB-E13064B
The standard is purified cortisol. This is a competitive kit. The plate is coated with specific antigen. The specific antibody is rabbit polyclonal antibody. The immunogen is modified by special modification.
HRP is conjugated with secondary antibody( goat anti-rabbit antibody).
Pls let me know if you have any further questions. Thank you.
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