Canine Cortisol ELISA Kit

Code CSB-E14303c
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Dog COR ELISA Kit was designed for the quantitative measurement of Dog COR protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

Target Name Cortisol
Alternative Names N/A
Abbreviation COR
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-98  
1:2 Average % 98  
Range % 92-106  
1:4 Average % 83  
Range % 80-87  
1:8 Average % 90  
Range % 83-96  
Recovery
The recovery of canine cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 93 87-98  
EDTA plasma (n=4) 97 92-105  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.171 0.185 0.178    
50 0.205 0.209 0.207    
12.5 0.317 0.293 0.305    
3.12 0.468 0.482 0.475    
0.78 0.821 0.878 0.850    
0.195 0.952 1.006 0.979    
0.049 1.208 1.200 1.204    
0 1.609 1.700 1.655    
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

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 Q&A
Q:

I bought a Cusabio Cortisol Saliva kit for dogs and it worked before. Do you have this for cat as well?
Actually I want to run a cat cortisol salivary kit but with high sensitivity.

A:
Thanks for your inquiry.Regarding your description,we have the following item available.
CSB-EQ027342 Cortisol (COR) ELISA kit -96T
The datasheet will be provided to you by e-mail.If you want to test cat samples,here advice you to do pre-testing.Pls let me know if you have any further questions. Thank you.
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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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