Goat Cortisol ELISA Kit

Instructions
Code CSB-E18048G
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Cortisol
Abbreviation COR
Species Capra hircus (Goat)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.05 ng/mL-200 ng/mL
Sensitivity 0.05 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of goat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 93  
Range % 86-97  
1:2 Average % 97  
Range % 92-102  
1:4 Average % 88  
Range % 85-90  
1:8 Average % 90  
Range % 84-95  
Recovery
The recovery of goat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 88-92  
EDTA plasma (n=4) 94 90-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.178 0.190 0.184    
50 0.256 0.267 0.262    
12.5 0.442 0.473 0.458    
3.12 0.674 0.672 0.673    
0.78 1.043 1.024 1.034    
0.195 1.321 1.310 1.316    
0.05 1.688 1.707 1.698    
0 2.049 1.924 1.987    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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 Q&A
Q:

I'm a PhD Candidate studying metabolic stress in dairy goats in the peripartum.
In total, 450 samples have to be analysed for each hormone, and analysis will be done in duplicate. Meaning I'll need a number of kits enough to run 900 tests.
Could you please send me a quote specifying the plate size (i.e. 96 well) and the number of free wells per plate. (i.e. 96 well minus the number of wells used for the standard curve).

A:
Thanks for your email!Regarding your request,we recommand this kit to you.
#CSB-E18048G,Goat Cortisol ELISA Kit *96T (If duplicate done,24 kits is advised;If not,only 12 kits is ok)
If you want to detect diary goat samples,we suggest you do pre-testing first.
Q:

I have a customer who is looking for Goat Cortisol (COR) ELISA Kit to test with feces samples. Please confirm that the kit is suitable for feces samples.
Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please provide your reference data for feces samples. If your lab didn’t test feces, please provide available reference data.
f. Please send the current manual.

A:
Thanks for your inquiry!
CSB-E18048G
This kit detects small molecule and it is based on competitive method.
The plate is coated with standard conjugate. The polyclonal antibody used in the kit is rabbit polyclonal antibody. The immunogen is modified standard. The secondary antibody used in the kit is goat-anti-rabbit antibody .The immunogen is rabbit IgG. Standard is purified small molecule.
We tested the mentioned sample stated in the manual..We suggest you follow the manual. We tested serum and plasma samples under 1:20 dilution before and the test value is 3-12.5ng/ml. The final concentration is 60--250ng/ml. You can refer to it.We can not ensure 100% on feces sample.If you want to test feces sample we suggest you do a pretest first if you want to test it.
And I will send you manual by email later.

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