Rat Cortisol ELISA Kit

Instructions
Code CSB-E05112r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name Cortisol
Abbreviation COR
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-98  
1:2 Average % 96  
Range % 92-104  
1:4 Average % 85  
Range % 81-88  
1:8 Average % 90  
Range % 83-97  
Recovery
The recovery of rat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-94  
EDTA plasma (n=4) 96 92-100  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.195 0.185 0.190    
50 0.336 0.321 0.329    
12.5 0.515 0.553 0.534    
3.12 0.913 0.982 0.948    
0.78 1.334 1.261 1.298    
0.195 1.663 1.656 1.660    
0.049 1.933 1.900 1.917    
0 2.326 2.402 2.364    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Q&A and Customer Reviews

 Q&A
Q:

Can we use processed brain tissue samples of rat for analysis?/

A:
Thanks for your inquiry.
We suggest you do a pretest first if you want to test brain tissues. Pls grind well when treating the samples.
Pls let me know if you have any further questions. Thank you.
 Customer Reviews

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