Rat Cortisol ELISA Kit

Code CSB-E05112r
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation COR
Protein Biological Process 1 Metabolism
Target Name Cortisol
Species Rattus norvegicus (Rat)
Sample Types serum, plasma
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rat cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-98  
1:2 Average % 96  
Range % 92-104  
1:4 Average % 85  
Range % 81-88  
1:8 Average % 90  
Range % 83-97  
The recovery of rat cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-94  
EDTA plasma (n=4) 96 92-100  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.195 0.185 0.190    
50 0.336 0.321 0.329    
12.5 0.515 0.553 0.534    
3.12 0.913 0.982 0.948    
0.78 1.334 1.261 1.298    
0.195 1.663 1.656 1.660    
0.049 1.933 1.900 1.917    
0 2.326 2.402 2.364    
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