Fish Cortisol ELISA Kit

Code CSB-E08487f
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Cortisol
Abbreviation COR
Species Fish
Sample Types serum, plasma, tissue homogenates
Detection Range 0.0023 ng/mL-10 ng/mL
Sensitivity 0.0023 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of fish Cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:20 Average % 90  
Range % 86-98  
1:40 Average % 96  
Range % 92-100  
1:80 Average % 82  
Range % 81-88  
1:160 Average % 95  
Range % 91-101  
The recovery of fish Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 87-96  
EDTA plasma (n=4) 98 92-105  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
10 0.207 0.196 0.202    
2.5 0.396 0.381 0.389    
0.625 0.531 0.575 0.553    
0.156 0.948 0.982 0.965    
0.039 1.326 1.390 1.358    
0.01 1.855 1.996 1.926    
0.0023 2.097 2.200 2.149    
0 2.367 2.302 2.335    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days


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