Human Cortisol ELISA Kit

Code CSB-E05111h
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Human COR ELISA Kit was designed for the quantitative measurement of Human COR protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.049 ng/mL-200 ng/mL and the sensitivity is 0.049 ng/mL.

Abbreviation COR
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 85  
Range % 81-89  
1:8 Average % 90  
Range % 83-96  
Recovery
The recovery of human cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-92  
EDTA plasma (n=4) 96 92-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.175 0.180 0.178    
50 0.246 0.231 0.239    
12.5 0.401 0.423 0.412    
3.12 0.661 0.682 0.672    
0.78 1.010 1.050 1.030    
0.195 1.316 1.306 1.311    
0.049 1.595 1.614 1.605    
0 1.862 1.922 1.892    
Materials provided
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
    • Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
    • Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
    • Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
    • HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
    • HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
    • Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
    • TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
    • Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 2 reviews

Submit a Review here

Sample type: Biological fluids(Saliva)

Sample species: Human

Review: Cortisol responses to the Trier Social Stress Test by bullying group. Cortisol was measured in nanograms per milliliter (ng/ml) and was log-transformed in this figure. The figure shows means for cortisol levels, for physical bullied, verbal bullied, relational bullied, the total bullied and nonbullied children.

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Human

Review: Cortisol levels in Experiment 2. Food, n = 21; Fasting, n = 24. b Cortisol levels in Experiment 4. Food, n = 22; Fasting, n = 23. Blue dots and red dots represent individuals in the food group and fasting group, respectively.

By Anonymous

 Q&A
Q:

I would like to know if corticosterone also affects to OD value.

A:
Thanks for your inquiry.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
Q:

I hope you would be able to advice me which kit would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on cortisol. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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