Human Cortisol ELISA Kit

Code CSB-E05111h
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase in six months till depleted. Apply now

Product Details

Abbreviation
COR
Species
Homo sapiens (Human)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates, saliva.
Detection Range
3.12 ng/ml-200 ng/ml.
Sensitivity
1.56 ng/ml.
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
 
Linearity
 
Recovery
 
Typical Data
 
Materials provided
    • A 96-well Assay plate -- The 96-well plate has been pre-coated with anti-human COR antibody.
    • Two vails Lyophilized Standard -- Dilute one of the standards at dilution series, read the OD values, and then draw a standard curve.
    • Anti-human COR Antibody (100 x concentrate) 1 x 60 μl-- Act as the detection antibody.
    • Antibody Diluent 1 x 10 ml -- Dilute the COR antibody solution.
    • HRP-conjugate (100 x concentrate) 1 x 120 μl-- Bind to the COR antibody, and HRP catalyzes the substrate A and B to elicit a chromogenic reaction.
    • HRP-conjugate Diluent 1 x 20 ml -- Dilute the HRP-conjugate solution.
    • Sample Diluent 2 x 20 ml -- Dilute the standard and sample solutions. Wash Buffer (25 x concentrate) 1 x 20 ml -- Wash away unbound or free substances.
    • TMB Substrate 1 x 10 ml -- HRP catalyzes the TMB, eliciting a chromogenic reaction.
    • Stop Solution 1 x 10 ml -- Stop the color reaction. The solution color immediately turns from blue to yellow.
    • Four Adhesive Strips (For 96 wells)
    • An Instruction manual
Materials not provided
    • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
    • An incubator that can provide stable incubation conditions up to 37°C±5°C.
    • Centrifuge
    • Vortex
    • Squirt bottle, manifold dispenser, or automated microplate washer
    • Absorbent paper for blotting the microtiter plate
    • 50-300ul multi-channel micropipette
    • Pipette tips
    • Single-channel micropipette with different ranges
    • 100ml and 500ml graduated cylinders
    • Deionized or distilled water
    • Timer
    • Test tubes for dilution
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Citations

Customer Reviews and Q&A

 Customer Reviews
Average Rating:
5.0 - 3 reviews

Submit a Review here

Sample type: Biological fluids(Saliva)

Sample species: Human

Review: Cortisol responses to the Trier Social Stress Test by bullying group. Cortisol was measured in nanograms per milliliter (ng/ml) and was log-transformed in this figure. The figure shows means for cortisol levels, for physical bullied, verbal bullied, relational bullied, the total bullied and nonbullied children.

By Anonymous

Sample type: Plasma (anticoagulant)

Sample species: Human

Review: Cortisol levels in Experiment 2. Food, n = 21; Fasting, n = 24. b Cortisol levels in Experiment 4. Food, n = 22; Fasting, n = 23. Blue dots and red dots represent individuals in the food group and fasting group, respectively.

By Anonymous

Sample type: Biological fluids(saliva)

Sample species: Human

Sample dilution: 1:4

Review: I used csb-E05111h to detect human salivary cortisol, the OD value is 1.5285, the concentration is 23.09, the overall standard curve of the kit is better and more stable.

By Anonymous

 Q&A
Q:

I would like to know if corticosterone also affects to OD value.

A:
Thanks for your inquiry.
This kit is specific to Cortisol.It has no correlation with corticosterone and the corticosterone does not affect OD value.
Q:

I hope you would be able to advice me which kit would
be suitable for me to purchase in order to conduct human salivary
ELISA assay on cortisol. Sample size is 60 and running in
duplicates.

A:
Thanks for your inquiry.
We have CSB-E05111h available. It is based on competitive method. It is suitable to test human salivary. Pls folow actual test result.
One 96T kit can test 43 samples if you run in duplicates.
CUSABIO guaranteed quality
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