Human Cortisol ELISA Kit

Code CSB-E05111h
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation COR
Protein Biological Process 1 Metabolism
Target Name Cortisol
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, saliva, tissue homogenates
Detection Range 0.049 ng/mL-200 ng/mL
Sensitivity 0.049 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human cortisol in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-99  
1:2 Average % 97  
Range % 92-103  
1:4 Average % 85  
Range % 81-89  
1:8 Average % 90  
Range % 83-96  
Recovery
The recovery of human cortisol spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 87-92  
EDTA plasma (n=4) 96 92-101  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
200 0.175 0.180 0.178    
50 0.246 0.231 0.239    
12.5 0.401 0.423 0.412    
3.12 0.661 0.682 0.672    
0.78 1.010 1.050 1.030    
0.195 1.316 1.306 1.311    
0.049 1.595 1.614 1.605    
0 1.862 1.922 1.892    
References
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

COR ELISA Kit

COR ELISA Kit for Bos taurus (Bovine)

COR ELISA Kit for Fish

COR ELISA Kit for Capra hircus (Goat)

COR ELISA Kit for Rattus norvegicus (Rat)

COR ELISA Kit for Canis lupus familiaris (Dog) (Canis familiaris)

COR ELISA Kit for Guinea Sus scrofa (Pig)

COR ELISA Kit for Mus musculus (Mouse)

COR ELISA Kit for Sus scrofa (Pig)

COR ELISA Kit for Monkey

COR ELISA Kit for Equus caballus (Horse)

COR ELISA Kit for Gallus gallus (Chicken)

COR ELISA Kit for General

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.