Bovine IgE ELISA Kit

Code CSB-E13028B
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgE
Target Name IgE
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 62.5 ng/mL-4000 ng/mL
Sensitivity 15.6 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 83-102  
1:2 Average % 93  
Range % 88-99  
1:4 Average % 94  
Range % 90-102  
1:8 Average % 90  
Range % 81-99  
Recovery
The recovery of bovine IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 97 90-104  
EDTA plasma (n=4) 95 87-106  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
4000 2.721 2.673 2.697 2.526  
2000 2.169 2.042 2.106 1.935  
1000 1.465 1.451 1.458 1.287  
500 0.928 0.906 0.917 0.746  
250 0.533 0.525 0.529 0.358  
125 0.390 0.378 0.384 0.213  
62.5 0.264 0.251 0.258 0.087  
0 0.173 0.168 0.171    
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