Mouse Immunoglobulin E,IgE ELISA Kit

Instructions
Code CSB-E07983m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Immunoglobulin E,IgE
Abbreviation IgE
Species Mus musculus (Mouse)
Sample Types serum, plasma
Detection Range 1.56 ng/mL-100 ng/mL
Sensitivity 0.39 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %89-95
1:2Average %102
Range %99-105
1:4Average %93
Range %87-96
1:8Average %99
Range %94-101
Recovery
The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9491-97
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.435 2.328 2.382 2.245
501.704 1.684 1.694 1.557
251.182 1.166 1.174 1.037
12.50.577 0.590 0.584 0.447
6.250.400 0.410 0.405 0.268
3.120.307 0.292 0.300 0.163
1.560.258 0.248 0.253 0.116
00.138 0.136 0.137  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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