Mouse Immunoglobulin E,IgE ELISA Kit

Code CSB-E07983m
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Immunoglobulin E,IgE
Abbreviation
IgE
Species
Mus musculus (Mouse)
Sample Types
serum, plasma
Detection Range
1.56 ng/mL-100 ng/mL
Sensitivity
0.39 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %93
Range %89-95
1:2Average %102
Range %99-105
1:4Average %93
Range %87-96
1:8Average %99
Range %94-101
Recovery
The recovery of mouse IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9491-97
EDTA plasma (n=4)9591-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
1002.435 2.328 2.382 2.245
501.704 1.684 1.694 1.557
251.182 1.166 1.174 1.037
12.50.577 0.590 0.584 0.447
6.250.400 0.410 0.405 0.268
3.120.307 0.292 0.300 0.163
1.560.258 0.248 0.253 0.116
00.138 0.136 0.137  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The mouse IgE ELISA Kit allows for the in vitro quantitative determination of IgE concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes mouse IgE. IgE is only found in mammals and is the least abundant immunoglobulin isotype in circulation. IgE is renowned for its functions in Th2 responses, protection against helminth parasites, and pathogenesis of allergy. IgE signals by binding to the high-affinity receptor FcεR1 and the low-affinity receptor FcεR2 or CD23. It plays an important role in acute allergic reactions and chronic inflammatory allergic diseases such as allergic rhinitis. IgE-FcεR1 signaling promotes inflammation and vascular remodeling. Type 1 hypersensitivity involves IgE-mediated activation of mast cells or basophils and subsequent release of preformed mediators including histamine, leukotrienes, and prostaglandins into the environment, finally causing the characteristic symptoms of allergy.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. IgE in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled IgE antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with IgE content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.

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