The mouse IgE ELISA Kit allows for the in vitro quantitative determination of IgE concentrations in serum, plasma, or tissue homogenates. This kit exclusively recognizes mouse IgE. IgE is only found in mammals and is the least abundant immunoglobulin isotype in circulation. IgE is renowned for its functions in Th2 responses, protection against helminth parasites, and pathogenesis of allergy. IgE signals by binding to the high-affinity receptor FcεR1 and the low-affinity receptor FcεR2 or CD23. It plays an important role in acute allergic reactions and chronic inflammatory allergic diseases such as allergic rhinitis. IgE-FcεR1 signaling promotes inflammation and vascular remodeling. Type 1 hypersensitivity involves IgE-mediated activation of mast cells or basophils and subsequent release of preformed mediators including histamine, leukotrienes, and prostaglandins into the environment, finally causing the characteristic symptoms of allergy.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. IgE in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled IgE antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with IgE content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.