Guinea pig Immunoglobulin E (IgE) ELISA kit

Code CSB-E12102Gp
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
Immunoglobulin E (IgE)
Abbreviation
IgE
Species
Cavia porcellus (Guinea pig)
Sample Types
serum, plasma, tissue homogenates
Detection Range
93.75 ng/mL-6000 ng/mL
Sensitivity
23.4 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IgE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %89
Range %82-96
1:2Average %94
Range %91-97
1:4Average %103
Range %96-107
1:8Average %98
Range %92-102
Recovery
The recovery of guinea pig IgE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 8982-96
EDTA plasma (n=4)106102-110
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
60002.755 2.654 2.705 2.615
30002.028 1.917 1.973 1.883
15001.195 1.174 1.185 1.095
7500.569 0.558 0.564 0.474
3750.322 0.312 0.317 0.227
187.50.254 0.243 0.249 0.159
93.750.148 0.144 0.146 0.056
00.090 0.089 0.090  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.
Description

This Guinea pig IgE ELISA Kit was designed for the quantitative measurement of Guinea pig IgE protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 93.75 ng/mL-6000 ng/mL and the sensitivity is 23.4 ng/mL .

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