Bovine Immunoglobulin A,IgA ELISA Kit

Code CSB-E12018B
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation IgA
Target Name Immunoglobulin A
Species Bos taurus (Bovine)
Sample Types serum, plasma, cell culture supernates, tissue homogenates_x000D_ _x000D_
Detection Range 0.39 μg/mL-25 μg/mL
Sensitivity 0.39 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 91  
Range % 86-99  
1:200 Average % 96  
Range % 92-101  
1:400 Average % 85  
Range % 81-87  
1:800 Average % 89  
Range % 83-94  
Recovery
The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 92 87-95  
EDTA plasma (n=4) 97 92-102  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
25 0.199 0.187 0.193    
12.5 0.325 0.329 0.327    
6.25 0.466 0.489 0.478    
3.12 0.731 0.760 0.746    
1.56 1.017 1.105 1.061    
0.78 1.310 1.410 1.360    
0.39 1.526 1.570 1.548    
0 2.125 2.162 2.144    
References
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